Electrical activities of bursting-firing neurons in epileptic network reestablishment of rat hippocampus
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2003.06.008
- Key Word:
- 癫痫网络;爆发式放电神经元;动作电位间期;强直电刺激;海马
Abstract： 本文探讨双侧海马(hippocampus,HPC)神经网络中爆发式放电神经元(bursting-firing neurons,BFN)的活动规律及其与海马癫痫网络重建的关系.实验用雄性SD大鼠140只(150～250 g),急性强直电刺激(60 Hz,2 s,0.4～0.6 mA)右后背HPC CAl区(acute tetanization of the posterior dorsal hippocampus,ATPDH),同步记录同侧或对侧前背HPC单位放电和深部电图;强直电刺激右前背HPC(acute tetanization of the anterior dorsal hippocampus,AT-ADH),同步记录双侧前背HPC单位放电.实验共记录了13.8%(19/138)双侧前背HPC的BFN,其中13个为刺激诱发性BFN,6个为自发性BFN.强直电刺激引起的诱发反应包括:(1)ATPDH明显调制同侧前背HPC的BFN,产生规则的节律性爆发式放电,刺激后串内动作电位间期(bursting interspike interval,BISI)减小(P＜0.001);(2)AT-PDH引起对侧前背HPC的BFN出现抑制后轻度调制效应,刺激后动作电位间期(interspike interval,ISI)增大(P＜0.001);(3)ATADH后易化对侧前背HPC的自发性BFN节律,增加ISI(P＜0.001)和IBI(P=0.01);(4)ATPDH诱导双侧前背HPC的BFN产生规则的节律性爆发式放电,伴有同步或非同步性网络癫痫的形成.上述实验结果提示,ATPDH沿同侧HPC长轴,跨大脑半球诱发前背HPC单个BFN的形成,其节律性爆发式放电与HPC癫痫网络的重建可能有关.
- [1]万业宏,菅忠,胡三觉,等.神经元阵发放电序列的非稳定周期轨道分析[J].2000,(3).
- [2]臧颖,韩丹,杨运煌,等.核磁共振检测大鼠早期癫痫源性脑损伤的动态发展特征[J].2002,(3).
- [3]韩丹,张先荣,唐岳枫,等.慢性电刺激诱发大鼠癫痫模型中核磁共振检测信号异常的非对称性与特征性行为发作的关系[J].2001,(3).
- [4]彭莉辉,吴鸿修,庄峻,等.神经电生理信号多道同步采集和分析系统[J].2001,(1).
- [5]Katz DM,Balkowiec A.Cellular mechanisms regulating activity-dependent release of native brain-derived neurotrophic factor from hippocampal neurons.[J].2002,22(23).
- [6]Kepecs A,Wang XJ,Lisman J.Bursting neurons signal input slope.[J].2002,22(20).
- [7]包新民,舒斯云著.大鼠脑立体定位图谱[M].1991.
- [8]Frysinger RC,Engel J Jr,Colder BW,etc.Interspike intervals during interictal periods in human temporal lobe epilepsy.[J].1996,719(1).
- [9]Beck H,Jiang Y,Becker A,etc.Upregulation of a T-type Ca2+ channel causes a long-lasting modification of neuronal firing mode after status epilepticus.[J].2002,22(9).
- [10]Gorter JanA,De-Graan-PierreN E,Aronica Eleonora,etc.Glutamate transporters alterations in the reorganizing dentate gyrus are associated with progressive seizure activity in chronic epileptic rats.[J].2002,442(4).
- [11]Dudek FE,Smith BN.Short- and long-term changes in CA1 network excitability after kainate treatment in rats.[J].2001,85(1).
- [12]Mody I,Wilson CL,Bragin A,etc.Local generation of fast ripples in epileptic brain.[J].2002,22(5).
- [13]Smith BN,Dudek FE.Network interactions mediated by new excitatory connections between CA1 pyramidal cells in rats with kainate-induced epilepsy.[J].2002,87(3).
- [14]D A, McCormick,D, Contreras.On the cellular and network bases of epileptic seizures.[J].2001,63.
- [15]Sanabria ER,Su H,Yaari Y.Initiation of network bursts by Ca2+-dependent intrinsic bursting in the rat pilocarpine model of temporal lobe epilepsy.[J].2001,532(Pt.1).
- [16]Collingridge G.Group I mGluRs modulate the pattern of non-synaptic epileptiform activity in the hippocampus.[J].2002,43(2).
- [17]Mamiya A,Csicsvari J,Harris KD,etc.Intracellular features predicted by extracellular recordings in the hippocampus in vivo.[J].2000,84(1).
- [18]B W, Colder,C L, Wilson,R C, Frysinger,etc.Neuronal synchrony in relation to burst discharge in epileptic human temporal lobes.[J].1996,75(6).