You Position: Home > Paper
Activating effect of ATP on NO/cGMP pathway in guinea pig cochlea
( views:187, downloads:8 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2003.06.007
- Key Word:
- 豚鼠;耳蜗;ATP;NO/cGMP途径
Abstract： 实验研究了豚鼠耳蜗中ATP和一氧化氮/环磷酸鸟苷途径(nitric oxide/cyclic guanosine monophosphate,NO/cGMP pathway)的关系.将40只耳廓反射灵敏的健康白色豚鼠随机分为5组,分别对其离体的耳蜗即刻灌流人工外淋巴基础液(artificial perilymph basic solution,APBS)以及溶于人工外淋巴基础液的ATP、一氧化氮合酶抑制剂左旋-NG-硝基精氨酸(L-NG-nitroarginine,L-NNA)+ATP、可溶性鸟苷酸环化酶抑制剂1H-[1,2,4]草酸重氮[4,3-a]喹恶啉(1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one,ODQ)+ATP和A-23187(Ca2+载体),收集耳蜗组织标本,利用放射免疫方法测定耳蜗组织中的cGMP的平均含量,比较各组之间耳蜗组织cGMP平均含量的差异.试验结果显示,向刚离体的耳蜗中灌流ATP和A-23187可以引起耳蜗组织中的cGMP含量升高,而灌流L-NNA和ODQ则可以抑制ATP所引起的耳蜗组织中cGMP含量的升高,提示在耳蜗组织中ATP可以通过升高细胞内Ca2+浓度的作用而激活NO/cGMP途径.从本实验结果可以提出假说:耳蜗中ATP从神经末梢释放,通过提高细胞内Ca2+的浓度,有激活NO/cGMP途径的作用,而NO/cGMP又能对ATP进行负反馈调节,两者共同调节耳蜗的生理功能,在耳蜗中存在ATP/Ca2+-NO/cGMP通路.
- [1]J D, Fessenden,J, Schacht.The nitric oxide/cyclic GMP pathway: a potential major regulator of cochlear physiology.[J].1998,118(1-2).
- [2]Mu�oz, D. J. B.,Thorne, P. R.,Housley, G. D.,etc.Extracellular adenosine 5'-triphosphate (ATP) in the endolymphatic compartment influences cochlear function[J].1995,90(1).
- [3]T, Nakagawa,N, Akaike,T, Kimitsuki,etc.ATP-induced current in isolated outer hair cells of guinea pig cochlea.[J].1990,63.
- [4]D, Dulon,R, Moataz,P, Mollard.Characterization of Ca2+ signals generated by extracellular nucleotides in supporting cells of the organ of Corti.[J].1993,14(3).
- [5]P P, Lefebvre,T R, Van De Water.Connexins, hearing and deafness: clinical aspects of mutations in the connexin 26 gene.[J].2000,32(1).
- [6]Shigemoto T,Ohmori H.Muscarinic agonists and ATP increase the intracellular Ca2+ concentration in chick cochlear hair cells.[J].1990,420(1).
- [7]Kikuchi, T.,Kimura, R. S.,Paul, D. L.,etc.Gap junctions in the rat cochlea: immunohistochemical and ultrastructural analysis[J].1995,191(2).
- [8]Heinrich UR,Maurer J,Gosepath K,etc.Immunoelectron microscopic localization of nitric oxide synthase III in the guinea pig organ of Corti.[J].1998,255(10).
- [9]Ren, T.,Nuttall, A. L.,Miller, J. M..ATP-induced cochlear blood flow changes involve the nitric oxide pathway[J].1997,112(1).
- [10]T, Yamashita,S, Ohnishi,M, Ohtani,etc.Effects of efferent neurotransmitters on intracellular Ca2+ concentration in vestibular hair cells of the guinea pig.[J].1993,500.
- [11]K, Ogawa,J, Schacht.Receptor-mediated release of inositol phosphates in the cochlear and vestibular sensory epithelia of the rat.[J].1993,69(1-2).
- [12]D, Dulon,P, Mollard,J M, Aran.Extracellular ATP elevates cytosolic Ca2+ in cochlear inner hair cells.[J].1991,2(2).
- [13]J. Lautermann,Wouter-Jan F. ten Cate,Petra Altenhoff,etc.Expression of the gap-junction connexins 26 and 30 in the rat cochlea[J].1998,294(3).
- [14]P, Franz,C, Hauser-Kronberger,P, B?ck,etc.Localization of nitric oxide synthase I and III in the cochlea.[J].1996,116(5).
- [15]Fessenden JD.,Schacht J..Localization of Soluble Guanylate Cyclase Activity in the Guinea Pig Cochlea Suggests Involvement in Regulation of Blood Flow and Supporting Cell Physiology[J].1997,45(10).
- [16]Matsunobu T,Schacht J.Nitric oxide/cyclic GMP pathway attenuates ATP-evoked intracellular calcium increase in supporting cells of the guinea pig cochlea.[J].2000,423(3).
- [17]Mammano F,Lagostena L,Ashmore JF,etc.Purinergic control of intercellular communication between Hensen's cells of the guinea-pig cochlea.[J].2001,531(Pt.3).
- [18]Katrin Gosepath,Ingolf Gath,Jan Maurer,etc.Characterization of nitric oxide synthase isoforms expressed in different structures of the guinea pig cochlea[J].1997,747(1).
- [19]J F, Ashmore,H, Ohmori.Control of intracellular calcium by ATP in isolated outer hair cells of the guinea-pig cochlea.[J].1990,428.