You Position: Home > Paper
Application of combination of capillary electrophoresis with laser-induced fluorescence:measurement of glutamate and arginine in PAG microdialytes of conscions morphine-withdrawal rats
( views:142, downloads:8 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.2003.05.020
- Key Word:
- 毛细管电泳;微透析;谷氨酸;精氨酸;导水管周围灰质
Abstract： 清醒动物脑微透析技术能够用于动态观察某一特定核团中生物活性物质变化及其与行为的关系,结合高效毛细管电泳-激光诱发荧光对衍生后的透析样品进行检测,使这一技术更趋完善.本实验用荧光素异硫氰酸酯(FITC)与痕量氨基酸样品进行衍生,通过适当增加衍生温度,能明显缩短衍生时间,衍生效果与传统的室温下衍生16 h无明显差别;进一步确定30℃水浴反应5 h是较理想的FITC与痕量氨基酸的衍生条件.在此优化衍生条件下,成功检测了清醒吗啡戒断大鼠脑导水管周围灰质(periaqueductal gray matter,PAG)微透析液中谷氨酸(glutamate,Glu)和精氨酸(arginine,Arg)含量变化.结果表明,非吗啡依赖和吗啡依赖大鼠脑PAG中L-精氨酸(L-arginine,L-Arg)和L-谷氨酸(L-glutamate,L-Glu)含量无明显差别,纳洛酮催促戒断后的第一个10 min内PAG中的L-Arg和L-Glu含量显著增加,分别比戒断前增加了63%和105%,10 min后含量逐渐下降,这种变化趋势与同时观察的吗啡戒断评分变化相一致.
- [1]张均田.现代药理实验方法[M].1997.
- [2]Tucci, S.,Rada, P.,Sepulveda, M. J.,etc.Glutamate measured by 6-s resolution brain microdialysis: capillary electrophoretic and laser-induced fluorescence detection application[J].1997,694(2).
- [3]R, Maldonado,L, Stinus,L H, Gold,etc.Role of different brain structures in the expression of the physical morphine withdrawal syndrome.[J].1992,261.
- [4]Denoroy L,Renaud B,Bert L,etc.Capillary Zone Electrophoresis with Laser-Induced Fluorescence Detection for the Determination of Nanomolar Concentrations of Noradrenaline and Dopamine - Application to Brain Microdialysate Analysis[J].1995,67(11).
- [5]M A, Bozarth.Physical dependence produced by central morphine infusions: an anatomical mapping study.[J].1994,18(3).
- [6]Vona MJ,Stiller CO,O'Connor WT,etc.Capillary electrophoresis with laser-induced fluorescence detection: a sensitive method for monitoring extracellular concentrations of amino acids in the periaqueductal grey matter.[J].1996,65(1).
- [7]Routledge C.,Stow JM.,Dawson LA.,etc.Analysis of Glutamate in Striatal Microdialysates Using Capillary Electrophoresis and Laser-Induced Fluorescence Detection[J].1995,700(1).
- [8]L A, Dawson,J M, Stow,A M, Palmer.Improved method for the measurement of glutamate and aspartate using capillary electrophoresis with laser induced fluorescence detection and its application to brain microdialysis.[J].1997,694(2).