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Developmental stage-dependence of cyclin B1 protein localization and gene expression in rabbit spermatogenesis
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.2002.05.009
- Key Word:
- cyclin B1;兔;精子发生;生精细胞发育阶段依赖性
Abstract： 利用原位杂交和免疫组化等方法, 研究兔精子发生过程中生精细胞cyclin B1 mRNA的表达和蛋白定位特点.结果显示: 兔生精上皮中cyclin B1 mRNA主要分布在初级精母细胞中, 直至圆形精子细胞仍然存在, 于精子细胞的变态过程中逐渐消失. 在伸长的精子细胞和精子中未检测出cyclin B1 mRNA.Cyclin B1蛋白在进入分裂期的精原细胞和精母细胞中表达; 在圆形精子细胞和伸长的精子细胞中呈现大量的cyclin B1蛋白.上述结果表明: 在兔精子发生过程中, cyclin B1 mRNA表达和蛋白定位具有发育阶段依赖性的特征.
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