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Effect of angiotensin Ⅱ on L-type calcium channel in ischemic ventricular myocytes of the guinea pig
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.2002.05.004
- Key Word:
- 血管紧张素Ⅱ;心肌缺血;全细胞膜片钳;L-型钙通道
Abstract： 实验研究了血管紧张素Ⅱ(Ang Ⅱ)对模拟缺血心室肌细胞L-型钙离子通道的作用.用胶原酶酶解法急性分离豚鼠心室肌细胞, 以全细胞膜片钳方法记录心室肌细胞的L-型钙电流(ICa.L).采用低氧、无糖、高乳酸和酸中毒综合方式模拟缺血液灌流, 造成心室肌细胞的模拟缺血, 并在缺血的基础上继续用含100 nmol/L Ang Ⅱ灌流细胞, 观察Ang Ⅱ对模拟缺血心室肌细胞钙离子通道的影响.实验结果显示: 模拟缺血时ICa.L峰值电流明显减小, 最大激活电压为0 mV; Ang Ⅱ能抵抗模拟缺血对ICa.L的抑制效应, 使ICa.L峰值电流增大, 并使最大激活电压左移至-10 mV.
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