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Effect of extracellular chloride concentration on deactivation kinetics of rat C lC-1 chloride channel

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Author:
No author available
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
3
DOI:
10.3321/j.issn:0371-0874.2002.03.006
Key Word:
ClC-1;氯离子通道;门控;爪蟾卵母细胞;动力学

Abstract´╝Ü 为探讨ClC-1通道的门控机制, 实验应用爪蟾卵母细胞异源性表达大鼠野生型ClC-1(WT rC lC-1)通道基因, 并使用双电极电压钳法记录通道电流.通过改变细胞外氯离子浓度, 采用双指数拟合的方法分析通道去激活电流, 对其去激活门控动力学特性进行了研究.结果表明 , 降低细胞外氯离子浓度可增加快速去激活电流成分, 减少慢速去激活成分; 同时, 慢速去激活和快速去激活电流的时间常数都显著减小, 说明细胞外氯离子浓度的改变可影响通道去激活动力学参数, 从而改变通道的门控过程.

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