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Changes in Gαq/11-mediated signal transduction pathway in the aorta of two kidney one clip hypertensive rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2001.06.007
- Key Word:
- 两肾一夹肾性高血压;Gαq/11亚单位;磷脂酶C;细胞外信号调节激酶 1/2
Abstract： 实验以两肾一夹肾性高血压大鼠为模型, 研究主动脉Gαq/11介导的细胞信号转导的变化及其意义.制备两肾一夹肾性高血压大鼠模型, 分别于术后第1、 2、 4和8周测定动脉血压, 用免疫印迹法检测主动脉组织中Gαq/11亚单位和细胞外信号调节激酶 1/2 (ERK1/2)含量, 测定磷脂酶C (phospholipase C, PLC)活性.结果显示高血压组大鼠于术后第2周血压开始升高; 主动脉Gαq/11和ERK1/2含量于术后第1周增加(分别为57.53%和40.16%), 并维持在高水平(P<0.01); 术后主动脉PLC活性亦增加(P<0.05).实验表明, 肾素依赖性高血压能引起大鼠主动脉Gαq/11介导的细胞信号转导系统激活, 并参与高血压的发生和发展.
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