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Inhibition of PKC and ERK1/2 in cultured rat vascular smooth muscle cells by angiotensin-(1-7)
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- Author:
- No author available
- Journal Title:
- ACTA PHYSILOGOCA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.2001.05.007
- Key Word:
- 血管紧张素-(1-7);蛋白激酶C;胞外调节蛋白激酶;血管平滑肌细胞
Abstract： 采用Western blot、氚-胸腺嘧啶(～3H-TdR)和氚-亮氨酸( ～3H-Leu )掺入等技术和方法, 用血管紧张素Ⅱ(Ang Ⅱ)和血管紧张素-(1-7)[Ang-(1-7)]刺激大鼠血管平滑肌细胞(VSMCs), 观察和分析Ang-(1-7)对VSMCs增殖及蛋白激酶C (PKC)和胞外调节蛋白激酶(ERK)表达的影响.Ang-(1-7)能明显抑制基础和Ang Ⅱ刺激下的VSMCs PKC-ζ和ERK1/2蛋白表达(P<0.01或P<0.05), 减少～3H-TdR 和～3H-Leu掺入量(P<0.01或P<0.05).结果提示, Ang-(1-7) 对VSMCs增殖有抑制作用, 这可能与影响PKC-ζ和ERK1/2蛋白表达有关.
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