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Roles of Gαq/11 mediated- and platelet-derived growth factor mediated-signal transduction pathways in rat aorta restenosis
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.2001.03.013
- Key Word:
- 再狭窄;G蛋白;血小板源生长因子受体;血管平滑肌细胞
Abstract： 采用大鼠主动脉球囊内皮剥脱术制备主动脉狭窄模型,观察Gαq/11和PDGF信号转导通路在大鼠主动脉球囊损伤后狭窄时血管平滑肌细胞(VSMC)增殖和迁移中的作用.实验分假手术组、损伤1 d组和损伤14 d组,观察形态学变化,检测血管紧张素转换酶(ACE)活性和主动脉磷脂酶C(PLC)活性,用免疫印迹法测定主动脉血小板源生长因子(PDGF)受体β和Gαq/11蛋白含量.结果显示:损伤1 d,主动脉内皮完全剥脱,VSMC无明显增殖和迁移,内膜无增厚.与假手术组比较,ACE活性增加382.7%(P＜0.01),PDGF受体β表达和PLC活性无明显变化,Gαq/11蛋白含量下降20.0%(P＜0.05).损伤14 d组,主动脉局部有新生内皮出现,中层VSMC大量增殖并向内膜下迁移,内膜显著增厚.ACE活性、PDGF受体β表达和PLC活性分别较假手术组升高420.2%(P＜0.01)、85.0%(P＜0.05)和186.2%(P＜0.05),Gαq/11蛋白表达下降33.1%(P＜0.01).结果提示,PDGF介导的信号转导通路可能是再狭窄时VSMC增殖的重要信号转导机制.
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