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Effects of berberine on intracellular calcium concentration in smooth muscle cells of guinea pig colon

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Author:
No author available
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
4
DOI:
10.3321/j.issn:0371-0874.2000.04.017
Key Word:
小檗碱;结肠平滑肌细胞;Fura-2;细胞内游离钙;乙酰胆碱;环匹阿尼酸;钙通道拮抗剂

Abstract´╝Ü 采用Ca2+荧光示踪剂Fura 2-AM和双波长荧光分光光度法, 观察小檗碱(berberine, Ber)对酶法分离的豚鼠结肠平滑肌细胞内游离钙([Ca2+]i)的影响并探讨其机制.在含1.5 mmol/L CaCl2的HEPES-Ringer缓冲液中, 豚鼠结肠平滑肌细胞[Ca2+]i为108±9.4 nmol/L (n=7), Ber对静息[Ca2+]i无明显影响, Ber呈浓度依赖性抑制, 60 mmol/L KCl引起的[Ca2+]i增高, IC50值为34.09 μmol/L.在含1.5 mmol/L Ca2+和无Ca2+的缓冲液中, 30、100 μmol/L Ber均显著抑制10 μmol/L ACh所诱发的[Ca2+]i的增高, 且有浓度依赖性; 同样Ber对环匹阿尼酸(CPA)所致的[Ca2+]i增高也有浓度依赖性抑制作用, 有钙和无钙条件下IC50分别为37.97 μmol/L和49.70 μmol/L.结果提示, Ber对结肠平滑肌细胞外Ca2+内流和细胞内钙释放均有抑制作用.

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