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Inhibition of ERK1/2 activity and c-fos mRNA after coronary artery balloon injury by intracoronary radiation in swine
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.2000.04.008
- Key Word:
- 血管内放射;血管成形术;再狭窄;c-fos;细胞外信号调节激酶1/2
Abstract： 本文旨在观察血管内放射对冠状动脉球囊扩张术后细胞外信号调节激酶1/2 (ERK1/2)及c-fos 基因表达的影响.实验对猪的左冠状动脉前降支或回旋支行球囊行过度扩张术, 术后即刻通过血管内放射治疗系统对猪冠状动脉损伤局部给予20 Gy的放射剂量, 分别在术后3 d 和30 d处死动物, 留取目标血管组织.通过反转录-聚合酶链反应定量检测血管内放射对球囊扩张术后血管组织c-fos mRNA的表达, 采用生化方法测定血管组织ERK1/2的活性.结果表明血管内放射治疗明显抑制了球囊扩张术后3 d ERK1/2活性和c-fos mRNA的表达(20.5%, P<0.01; 47.7%, P<0.05), 而对球囊扩张术后30 d的ERK1/2活性和c-fos mRNA表达无显著影响.这提示ERK1/2和c-fos可能参与了血管内放射抑制球囊扩张术后早期再狭窄过程.
- [1]C. Indolfi,E. V. Avvedimento,A. Rapacciuolo,etc.In vivo gene transfer: prevention of neointima formation by inhibition of mitogen-activated protein kinase kinase[J].1997,92(6).
- [2]Kato Y,Chung HO,Kato T.Molecular cloning of c-jun and c-fos cDNAs from porcine anterior pituitary and their involvement in gonadotropin-releasing hormone stimulation.[J].1996,119(1).
- [3]J A, Condado,R, Waksman,O, Gurdiel,etc.Long-term angiographic and clinical outcome after percutaneous transluminal coronary angioplasty and intracoronary radiation therapy in humans.[J].1997,96.
- [4]Joseph G. Wiedermann,Charles Marboe,Howard Amols,etc.Intracoronary irradiation markedly reduces restenosis after balloon angioplasty in a porcine model[J].1994,23(6).
- [5]Y, Zou,Y, Hu,B, Metzler,etc.Signal transduction in arteriosclerosis: mechanical stress-activated MAP kinases in vascular smooth muscle cells (review).[J].1998,1(5).
- [6]P S, Teirstein,V, Massullo,S, Jani,etc.Catheter-based radiotherapy to inhibit restenosis after coronary stenting.[J].1997,336.
- [7]Hiroyuki Koyama,Eric Olson,Frank Dastvan,etc.Cell Replication in the Arterial WallActivation of Signaling Pathway Following In Vivo Injury[J].1998,82(6).
- [8]Force T,Bonventre JV.Growth factors and mitogen-activated protein kinases.[J].1998,31(1 Pt.2).
- [9]R J, Davis.The mitogen-activated protein kinase signal transduction pathway.[J].1993,268(20).
- [10]J M, Pyles,K L, March,M, Franklin,etc.Activation of MAP kinase in vivo follows balloon overstretch injury of porcine coronary and carotid arteries.[J].1997,81.
- [11]Lorraine Yau,Peter Zahradka.Immunodetection of activated mitogen-activated protein kinase in vascular tissues[J].1997,172(1).
- [12]C A, Lange-Carter,C M, Pleiman,A M, Gardner,etc.A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.[J].1993,260.
- [13]Y, Hu,L, Cheng,B W, Hochleitner,etc.Activation of mitogen-activated protein kinases (ERK/JNK) and AP-1 transcription factor in rat carotid arteries after balloon injury.[J].1997,17.