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Cardiocyte hypertrophy induced by cultured neonatal rat ventricular fibroblast conditioned growth medium
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2000.01.008
- Key Word:
- 成纤维细胞;心肌细胞;肥大;细胞培养;心脏
Abstract： 利用新生大鼠心室成纤维细胞条件培养液孵育心肌细胞,证实成纤维细胞条件培养液能明显增大心肌细胞的表面积,增加心肌细胞的蛋白含量和[3H]-亮氨酸([3H]-Leu)的掺入,上述作用以第3天的条件培养液作用最强,具有剂量依赖性.ETA受体拮抗剂BQ123能部分阻断成纤维细胞条件培养液促进心肌细胞肥大的作用,而AT1受体拮抗剂CV11974和α-肾上腺素受体拮抗剂regitin却无此效果. 结果提示: 成纤维细胞条件培养液中含有促使心肌细胞肥大的物质,这些物质可能是内皮素(ET-1)等. 百日咳毒素(PTX)和staurosporine也能部分阻断成纤维细胞条件培养液促进心肌细胞肥大作用,表明培养液的促肥大作用可能与PTX敏感的G蛋白及PKC有关.
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