You Position: Home > Paper
Effects of c-erbB2 on the hCG-induced progesterone production of rat luteal cells
( views:164, downloads:11 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2000.01.005
- Key Word:
- 黄体细胞;c-erbB2;反义寡脱氧核苷酸;孕酮
Abstract： 采用离体细胞体外孵育法,研究反义c-erbB2寡脱氧核苷酸(antisense c-erbB2 ODN)对大鼠黄体细胞hCG 诱导的孕酮分泌的影响,及其与外源性cAMP和Ca2+以及蛋白抑制剂放线菌酮(CYX)之间的关系. 结果表明,反义 c-erbB2以剂量相关方式抑制黄体细胞hCG诱导的孕酮的产生,同时使c-erbB2蛋白染色阳性的黄体细胞百分数下降,无义tat ODN没有相应的作用. 10-4 mol/L的二丁酰cAMP能明显反转反义c-erbB2 ODN对孕酮产生和c-erbB2表达的抑制作用,钙离子通道阻断剂维拉帕米和蛋白抑制剂CYX 对此抑制作用有协同效应. 该实验说明c-erbB2参于hCG诱导黄体细胞生孕酮作用.
- [1]Y, Piontkewitz,K, Sundfeldt,L, Hedin.The expression of c-myc during follicular growth and luteal formation in the rat ovary in vivo.[J].1997,152(3).
- [2]V, Narasimhan,O, Hamill,R A, Cerione.The effects of the normal and oncogenic forms of the neu tyrosine kinase, and the corresponding forms of an immunoglobulin E receptor/neu tyrosine kinase fusion protein, on Xenopus oocyte maturation.[J].1992,303(2-3).
- [3]Mark Pennybacker,Brian Herman.Follicle-stimulating hormone increases c-fos mRNA levels in rat granulosa cells via a protein kinase C-dependent mechanism[J].1991,80(1-3).
- [4]L, Wu,A, Wu,K, Jiang.[Effect of antisense c-erbB2 on biologic behaviour and chemotherapeutic drug sensitivity in human ovarian cancer cells].[J].1996,31(3).
- [5]B C, Delidow,J P, Lynch,B A, White,etc.Regulation of proto-oncogene expression and deoxyribonucleic acid synthesis in granulosa cells of perifused immature rat ovaries.[J].1992,47(3).
- [6]N. Miosge,Wolfgang Schneider,Werner Götz,etc.The oncoproteins c-erb-B2, c-fos and the tumour suppressor protein p53 in human embryos and fetuses[J].1997,195(4).
- [7]Putowski LT,Skrzypczak M,Zielewicz J,etc.The relevance of c-myc to the physiology of the human ovary.[J].1997,11(1).
- [8]Martha E. Shelley,Amjad Hossain,Paul G. McDonough,etc.Differential c-jun gene expression with tonically administered steroids in rat ovary and uterus[J].1994,170(5).
- [9]B C, Delidow,B A, White,J J, Peluso.Gonadotropin induction of c-fos and c-myc expression and deoxyribonucleic acid synthesis in rat granulosa cells.[J].1990,126(5).