You Position: Home > Paper
EFFECTS OF BONE MARROW ENDOTHELIAL CELL CONDITIONED MEDIUM AND ITS ULTRAFILTRATION-PREPARED COMPONENTS ON THE GROWTH OF CFU-GM
( views:83, downloads:0 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.1999.06.007
- Key Word:
- 骨髓内皮细胞;条件培养液;生长因子;生长抑制因子;粒巨噬系祖细胞
Abstract： 本研究通过传代培养小鼠骨髓内皮细胞系细胞, 收集无血清条件培养液(mBMEC-CM), 将其作多级串联超滤, 获得分子量大于10、 3～10、 1～3、 0.5～1 kD和小于0.5 kD超滤组分.进行粒巨噬系造血祖细胞集落形成试验, 检测了它们的作用.mBMEC-CM 和3～10 kD组分对粒巨噬系祖细胞(CFU-GM)生长未见明显影响, 而分子量大于10 kD和0.5～1 kD组分促进CFU-GM的增殖, 分子量1～3 kD和小于0.5 kD组分则抑制CFU-GM的增殖.这4种超滤组分对CFU-GM生长的效应均有剂量依赖性.这些结果提示, 在体外培养条件下, 小鼠骨髓内皮细胞分泌若干种活性成分, 分别对CFU-GM的生长起促进或抑制作用.
- [1]O, Ohneda,V L, Bautch.Murine endothelial cells support fetal liver erythropoiesis and myelopoiesis via distinct interactions.[J].1997,98(4).
- [2]I S, Weimar,N, Miranda,E J, Muller,etc.Hepatocyte growth factor/scatter factor (HGF/SF) is produced by human bone marrow stromal cells and promotes proliferation, adhesion and survival of human hematopoietic progenitor cells (CD34+).[J].1998,26.
- [3]S E, Jacobsen,F W, Ruscetti,M, Ortiz,etc.The growth response of Lin-Thy-1+ hematopoietic progenitors to cytokines is determined by the balance between synergy of multiple stimulators and negative cooperation of multiple inhibitors.[J].1994,22.
- [4]K, Thalmeier,P, Meissner,G, Reisbach,etc.Constitutive and modulated cytokine expression in two permanent human bone marrow stromal cell lines.[J].1996,24.
- [5]Qi Ru Wang,Yan Yan,Bao He Wang,etc.Long-term culture of murine bone-marrow-derived endothelial cells[J].1998,34(6).
- [6]F. M. Gibson,J. Scopes,S. Daly,etc.Haemopoietic growth factor production by normal and aplastic anaemia stroma in long-term bone marrow culture[J].1995,91(3).
- [7]S, Rafii,F, Shapiro,R, Pettengell,etc.Human bone marrow microvascular endothelial cells support long-term proliferation and differentiation of myeloid and megakaryocytic progenitors.[J].1995,86.
- [8]G, Almeida-Porada,J L, Ascens?o.Isolation, characterization, and biologic features of bone marrow endothelial cells.[J].1996,128(4).