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MELATONIN DECREASES THE RELEASE OF AMINO ACID TRANSMITTERS IN HYPOXIC RAT CORTICAL SLICE
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.1999.04.014
- Key Word:
- 褪黑素;低氧;脑片;兴奋性氨基酸zzzzzzzz
Abstract： 为研究褪黑素对低氧引起大鼠大脑皮层脑片氨基酸释放变化的影响, 利用反相高效液相色谱结合荧光检测法, 测定了孵育液中氨基酸类神经递质的含量. 低氧条件为通入91.6% N2和8.4% O2的混合气体. 低氧30 min时, 大鼠大脑皮层脑片孵育液中, 氨基酸类神经递质天冬氨酸、谷氨酸、谷氨酰胺、甘氨酸、牛磺酸和γ-氨基丁酸的含量显著增加, 其含量分别是正常氧组的240.4%, 334.3%, 200.6%, 210.4%, 168.6% 和263.9%, 与正常氧组比较 P<0.01. 予以褪黑素可以显著降低兴奋性氨基酸的释放, 其中天冬氨酸和谷氨酸的含量分别是低氧组的55.1% 和57.0%. 结果表明, 褪黑素具有降低低氧所致的脑片释放兴奋性氨基酸的作用.
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