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Vasoactive intestinal peptide and epidermal growth factor upregulates bcl-2 gene expression in bron-chial epithelial cells
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.1999.04.009
- Key Word:
- 支气管上皮细胞;bcl-2基因;c-myc基因;表皮生长因子;血管活性肠肽
Abstract： 为探索肺内调节肽血管活性肠肽(VIP)和表皮生长因子(EGF)抗氧化保护的基因机制, 用逆转录聚合酶链式反应(RT-PCR)及Southern blot杂交等方法检测原代培养的兔支气管上皮细胞(BEC)内bcl-2和c-myc基因的表达情况, 观察VIP、EGF及热应激对这两个基因表达的影响.结果显示:(1)基础情况下BEC内有bcl-2和c-myc基因的低水平表达; (2) EGF和VIP均明显增强bcl-2和c-myc的转录, EGF的作用更强, 而热应激无明显效应;(3) bcl-2和c-myc两者的转录有显著相关性.上述结果提示, VIP和EGF等肺内调节肽可通过上调bcl-2基因表达增强支气管上皮细胞的抗氧化能力;c-myc基因的编码产物可能是bcl-2基因转录的上游调节因子.
- [1]A, Fanidi,E A, Harrington,G I, Evan.Cooperative interaction between c-myc and bcl-2 proto-oncogenes.[J].1992,359(6395).
- [2]D M, Hockenbery,Z N, Oltvai,X M, Yin,etc.Bcl-2 functions in an antioxidant pathway to prevent apoptosis.[J].1993,75(2).
- [3]Reed J. C..Bcl-2 and the regulation of programmed cell death[J].1994.
- [4]Tsujimoto Y.,Croce C. M..Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma.[J].1986.
- [5]秦晓群.热应激对支气管上皮细胞的抗臭氧细胞保护作用[J].1996,(3).
- [6]秦晓群,孙秀泓,罗自强,等.表皮生长因子对培养的气道上皮细胞抗臭氧损伤的保护作用[J].1996,(2).
- [7]秦晓群.血管活性肠肽对兔支气管上皮细胞抗臭氧损伤的保护作用[J].1998,(3).