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ALTERATION OF RYANODINE RECEPTORS IN CARDIAC SARCOPLASMIC RETICULUM AND NUCLEAR ENVELOPE OF RATS DURING SEPSIS
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.1999.03.017
- Key Word:
- 败血症;肌浆网;核被膜;ryanodine受体
Abstract： 为观察败血症时心肌肌浆网(SR)和核被膜(NE)的ryanodine受体的变化,采用结扎及穿刺盲肠(CLP)制作败血症动物模型,用密度梯度离心分离SR和NE,用放射配体结合法研究ryanodine受体的特征.结果表明,大鼠早期败血症(CLP后9h)时,SR的ryanodine受体的最大结合(Bmax)增加23%,NE的ryanodine受体的Bmax则增加1倍,二者比值降低39%(P<0.01); 在晚期败血症(CLP后18h)时,SR ryanodine受体的Bmax降低了38%,NE的ryanodine受体的Bmax增加1.6倍,二者比值降低76%; SR和NE ryanodine受体的离解常数无显著改变.败血症时,SR ryanodine受体早期上调,晚期下调,而NE ryanodine受体均上调,这些变化可能与休克时相有关.
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