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EFFECT OF ENKEPHALIN ON GLIAL NEUROTROPHIC FUNCTION RELATED TO THE REDUCTION OF NITRIC OXIDE PRODUCTION
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.1999.03.015
- Key Word:
- 脑啡肽;胶质细胞-神经元共培养;GAP-43;NO
Abstract： 本文在SD大鼠大脑皮层胶质细胞-神经元共培养模式上,以神经元存活、突起生长、生长相关蛋白-43(growth associated protein-43,GAP-43)mRNA的表达为指标,观察了脑啡肽对胶质细胞神经营养作用的影响,并对其机理作了初步探讨.结果表明,经脑啡肽处理的胶质细胞能使神经元的存活计数增加28%(P<0.05),单个神经元突起总长度增加11%(P<0.05),最长突起长度增加16%(P<0.05),GAP-43 mRNA的表达增加26%(P<0.05).然后又观察了脑啡肽(10-6～10-12mol/L)对培养胶质细胞生成一氧化氮(NO)的影响.结果表明,浓度为10-8,10-10mol/L的脑啡肽能明显抑制其生成(P<0.05).结果提示,脑啡肽可能增强胶质细胞的神经营养作用,其机制之一可能是通过抑制胶质细胞NO的生成.
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