You Position: Home > Paper
ESTABLISHMENT OF AN APOPTOSIS MODEL OF VASCULAR SMOOTH MUSCLE CELLS INDUCED BY ULTRAVIOLET-C RADIATION IN VITRO
( views:212, downloads:16 )
- Author:
- No author available
- Journal Title:
- ACTA PHTYSIOLOGOCA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.1999.02.019
- Key Word:
- 平滑肌细胞;细胞凋亡;紫外线;动脉粥样硬化
Abstract： 应用常规细胞培养超净台紫外消毒灯(220 W, 220 V, 50 Hz)发射的UV-C波段的紫外光源(254 nm), 垂直照射距离其10 cm 处的大鼠主动脉平滑肌细胞(smooth muscle cells, SMCs), 发现经照射后细胞出现典型的凋亡形态学改变, 如细胞变圆、染色质浓缩、细胞膜出泡、出现凋亡小体等;细胞面积、核面积及核/胞面积比均显著降低;且提取细胞DNA的琼脂糖凝胶电泳呈现梯状图谱.从形态学和生化指标方面证明了UV-C照射可诱导体外血管SMCs凋亡.此方法可作为进一步研究SMCs凋亡的内在调控机制的一个简便、稳定、可靠的模型.
- [1]S C, Wright,Q S, Wei,J, Zhong,etc.Purification of a 24-kD protease from apoptotic tumor cells that activates DNA fragmentation.[J].1994,180(6).
- [2]L E, French,A, Wohlwend,A P, Sappino,etc.Human clusterin gene expression is confined to surviving cells during in vitro programmed cell death.[J].1994,93(2).
- [3]Sergei N. Orlov,Than-Vinh Dam,Johanne Tremblay,etc.Apoptosis in Vascular Smooth Muscle Cells: Role of Cell Shrinkage[J].1996,221(3).
- [4]K, Servomaa,T, Ryt?maa.UV light and ionizing radiations cause programmed death of rat chloroleukaemia cells by inducing retropositions of a mobile DNA element (L1Rn).[J].1990,57(2).
- [5]Maytin EV,Kane KS.Ultraviolet B-induced apoptosis of keratinocytes in murine skin is reduced by mild local hyperthermia.[J].1995,104(1).
- [6]Majno G,Joris I.Apoptosis, oncosis, and necrosis. An overview of cell death.[J].1995,146(1).
- [7]Godar DE.,Lucas AD..Spectral Dependence of UV-Induced Immediate and Delayed Apoptosis - the Role of Membrane and Dna Damage[J].1995,62(1).
- [8]D. E. HOLLIS,A. SCHEIBNER.Ultrastructural changes in epidermal Langerhans cells and melanocytes in response to ultraviolet irradiation, in Australians of Aboriginal and Celtic descent[J].1988,119(1).
- [9].Ultraviolet Radiation and Plants: Burning Questions[J].1992,4(11).
- [10]L S, Bazar,H J, Deeg.Ultraviolet B-induced DNA fragmentation (apoptosis) in activated T-lymphocytes and Jurkat cells is augmented by inhibition of RNA and protein synthesis.[J].1992,20(1).