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Inhibitory effects of piperine on proliferation,migration,and invasion of human colon cancer SW480 cells
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 12
- DOI:
- 10.11569/wcjd.v29.i12.639
- Key Word:
- 结肠癌;胡椒碱;增殖;迁移;侵袭;自噬
Abstract： 背景 最近许多报道显示胡椒碱(piperine,PIP)具有抗癌活性,而其在结肠癌中的具体作用及生物学机制的研究并不完善.目的 探究PIP对人结肠癌SW480细胞增殖、迁移和侵袭能力的影响及潜在机制.方法 分别用0(空白对照)、5、10和20 μg/mL的PIP处理SW480细胞24 h,采用细胞计数试剂盒8(cell counting kit-8,CCK-8)法检测细胞活性;采用5-乙炔基-2'脱氧尿嘧啶核苷(5-Ethynyl-2'-deoxyuridine,EdU)染色法检测细胞增殖;采用Transwell法检测细胞迁移和侵袭;采用蛋白质免疫印迹(Western blotting)法检测细胞中微管相关蛋白轻链3(microtubule-associated light chain 3,LC3)、p53、B细胞淋巴瘤/白血病-2(B cell lymphoma/lewkmia-2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、劈裂的半胱氨酸天冬氨酸蛋白酶-3(cleaved cysteine aspartate proteinase-3,Cleaved caspase 3)蛋白表达水平.结果 PIP浓度依赖性抑制细胞活力、增殖、迁移和侵袭.Western blotting结果显示,与空白对照组相比,PIP能升高SW480细胞中p53、Bax、Cleaved caspase 3的表达并降低Bcl-2的表达.结论 PIP可抑制SW480细胞增殖、迁移和侵袭,其机制可能与上调p53信号通路和诱导自噬性死亡相关.
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