Clinical significance of changes of TLR4/MyD88/NF-κB signaling pathway in peripheral blood mononuclear cells of gastric cancer patients with Helicobacter pylori infection
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 6
- DOI:
- 10.11569/wcjd.v29.i6.319
- Key Word:
- 幽门螺杆菌感染;胃癌;病理特征;外周血;单核细胞;TLR4/MyD88/NF-κB信号通路
Abstract： 背景幽门螺杆菌(Helicobacter pylori,H.pylori)与胃癌的相关性已经得到了世界范围内的普遍认可,而TLR4/MyD88/NF-κB信号通路相关因子在胃癌患者中明显存在异常表达,因此与胃癌的发生及发展存在密切关系,但其是否与胃癌患者H.pylori感染之间也存在相关性,临床相关研究较少,值得进一步研究.目的探究H.pylori感染性胃癌患者外周血单核细胞TLR4/MyD88/NF-κB信号通路的变化及其临床意义.方法选取2017-10/2020-10于湖州市第三人民医院收治的80例胃癌患者作为研究组,另外选择同期来我院进行体检且体检结果合格的50例健康者作为对照组.比较两组受试者、不同病理特征胃癌患者以及胃癌H.pylori感染患者外周血单核细胞中TLR-4、核因子κB (NF-κB)和髓样分化因子(MyD88)蛋白表达水平,并分析胃癌患者TLR-4、NF-κB和MyD88蛋白水平与H.pylori感染的相关性.结果与对照组相比较,研究组TLR-4、NF-κB、MyD88表达均明显偏高,差异均有统计学意义(P＜0.05);胃癌患者不同临床分期、是否出现淋巴结转移和不同浸润深度的TLR4/MyD88/NF-κB信号通路相关分子表达比较,差异均有统计学意义(P＜0.05);而不同性别、年龄的TLR4/MyD88/NF-κB信号通路相关分子表达比较,差异无统计学意义(P＞0.05);与H.pylori感染阴性相比较,H.pylori感染阳性TLR-4、NF-κB、MyD88表达均明显偏高,差异均有统计学意义(P＜0.05);Pearson检验发现,H.pylori感染与TLR-4、NF-κB、MyD88表达之间有正相关(r=0.726、0.684、0.631,P＜0.01).结论胃癌患者外周血单核细胞中的TLR4/MyD88/NF-κB信号通路相关分子表达水平均显著升高,且不同病理特征及参数分组下的TLR4/MyD88/NF-κB信号通路相关分子表达水平存在显著差异,推测H.pylori感染可能通过诱发TLR4/MyD88/NF-κB信号通路异常表达参与了胃癌的发生及发展.
- [1]张子杰,袁征,闫振宇.肝细胞癌组织中TLR4、MyD88、NF-kB表达量及其与临床病理特征的相关性分析[J].2017,(4).
- [2]万晶晶.胃癌病因学的研究进展[J].2014,(14).
- [3]陈锦霞,单保恩,李清靖,等.IL-17及其相关分子在胃癌组织中的表达和相关性分析[J].2019,(6).
- [4]K, Fujitani,M, Ando,K, Sakamaki,etc.Multicentre observational study of quality of life after surgical palliation of malignant gastric outlet obstruction for gastric cancer.[J].2017,1(6).
- [5]Hassan, Noha F.,Nada, Somaia A.,Hassan, Azza,etc.Saroglitazar Deactivates the Hepatic LPS/TLR4 Signaling Pathway and Ameliorates Adipocyte Dysfunction in Rats with High-Fat Emulsion/LPS Model-Induced Non-alcoholic Steatohepatitis[J].2019,42(3).
- [6]李斌,李玉民,郭继武,等.幽门螺杆菌与胃癌相关机制的研究进展[J].2015,(7).
- [7]Li-Ping Liu,Xi-Ping Sheng,Tian-Kui Shuai,etc.Helicobacter pylori promotes invasion and metastasis of gastric cancer by enhancing heparanase expression[J].2018,(40).
- [8]Ali Pormohammad,Naser Mohtavinejad,Pourya Gholizadeh,etc.Global estimate of gastric cancer in Helicobacter pylori-infected population: A systematic review and meta-analysis[J].2019,234(2).
- [9]Rajput Sandeep,Boral Debasish,Ran Sophia.Abstract 1700: TLR4 as a novel determinant of paclitaxel response in metastatic breast cancer[J].2011.
- [10]Wang EL,Nakasono,Qian ZR.High expression of Toll-like receptor 4/myeloid differentiation factor 88 signals correlates with poor prognosis in colorectal cancer.[J].2010,102(5).
- [11]Paula, Díaz,Manuel, Valenzuela Valderrama,Jimena, Bravo,etc.Helicobacter pylori and Gastric Cancer: Adaptive Cellular Mechanisms Involved in Disease Progression.[J].2018,9(Spec ).
- [12]Cong, Li,Yang, Shuting,Zhang, Yong,etc.DFMG attenuates the activation of macrophages induced by co-culture with LPC-injured HUVE-12 cells via the TLR4/MyD88/NF-kappa B signaling pathway[J].2018,41(5).
- [13]Ki Hyung, Kim,Moo Sung, Jo,Dong Soo, Suh,etc.Expression and significance of the TLR4/MyD88 signaling pathway in ovarian epithelial cancers.[J].2012,10.
- [14]Hayet, Rafa,Sarra, Benkhelifa,Sonia, AitYounes,etc.All-Trans Retinoic Acid Modulates TLR4/NF-κB Signaling Pathway Targeting TNF-α and Nitric Oxide Synthase 2 Expression in Colonic Mucosa during Ulcerative Colitis and Colitis Associated Cancer.[J].2017,2017(Spec ).
- [15]Hu, Anla,Li, Li,Hu, Chuanlai,etc.Serum Concentrations of 15 Elements Among Helicobacter Pylori-Infected Residents from Lujiang County with High Gastric Cancer Risk in Eastern China[J].2018,186(1).
- [16]Zhixue, Wang,Bing, Dong,Zifang, Feng,etc.A study on immunomodulatory mechanism of Polysaccharopeptide mediated by TLR4 signaling pathway.[J].2015,16.
- [17]Chung-Yi,Chen,Chiu-Li,Kao,Chi-Ming,Liu.The Cancer Prevention, Anti-Inflammatory and Anti-Oxidation of Bioactive Phytochemicals Targeting the TLR4 Signaling Pathway.[J].1909,19(9).
- [18]Hidekazu, Tsukamoto,Lopa, Mishra,Keigo, Machida.Alcohol, TLR4-TGF-β antagonism, and liver cancer.[J].2014,8 Suppl 2.