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Down-regulation of CIC-3 enhances chemosensitivity of colorectal cancer cells to oxaliplatin by inhibiting autophagy
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 24
- DOI:
- 10.11569/wcjd.v28.i24.1235
- Key Word:
- ClC-3;细胞自噬;结直肠癌;奥沙利铂;化疗敏感性
Abstract： 背景 电压门控氯通道3(voltage-gated chloride channel 3,ClC-3)可参与调节卵巢癌、宫颈癌和肺癌的化疗敏感性的调节,而其在结直肠癌(colorectal cancer,CRC)的奥沙利铂化疗中的作用和机制尚不清楚.目的 探究CRC细胞中ClC-3对奥沙利铂化疗敏感性的影响,以及细胞自噬在其中发挥的作用.方法 用免疫组化染色观察ClC-3和微管相关蛋白1Ⅱ轻链3(microtubule-associated protein 1Ⅱ light chain 3,LC3-Ⅱ)在CRC耐药和敏感组织中的表达.用Western blot检测ClC-3和LC3-Ⅱ在CRC HT-29细胞以及奥沙利铂耐药细胞(HT-29/L-OHP)中的表达.用ClC-3 siRNA分别转染HT-29细胞和HT-29/L-OHP细胞后,CCK-8法检测细胞对奥沙利铂的敏感性变化;用Cyto-ID染色观察自噬点形成;用Western blot检测自噬相关蛋白Beclinl、LC3-Ⅰ、LC3-Ⅱ和p62的表达.用自噬激动剂雷帕霉素处理已转染ClC-3 siRNA的HT-29细胞和HT-29/L-OHP细胞后,用CCK-8法和Western blot分别检测细胞对奥沙利铂的敏感性变化以及Beclin1、LC3-Ⅰ、LC3-Ⅱ、p62和ClC-3蛋白的表达.
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