Biological significance of expression of circ_0001785 and miR-330-5p in colorectal cancer
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 17
- DOI:
- 10.11569/wcjd.v28.i17.865
- Key Word:
- circ_0001785;miR-330-5p;结直肠癌;增殖;迁移;侵袭
Abstract： 背景 结直肠癌(colorectal cancer, CRC)发病机制尚未cular RNA,circRNA)在CRC等肿瘤中表达异常并可调控细胞增殖、迁移及侵袭等生物学过程,但关于其具体作用机制尚未完全阐明,因而探寻新型circRNA分子并探究其可能作用机制对进一步揭示CRC发病机制具有重要意义.目的 探讨circ_0001785与miR-330-5p在CRC中的表达及其生物学意义.方法 采用qRT-PCR法检测CRC组织、癌旁组织中circ_ 0001785、miR-330-5p的表达量;采用Pearson法检测CRC中circ_0001785与miR-330-5p的相关性;根据circ_0001785、miR-330-5p表达量的平均值将患者分为circ_0001785高表达组41例、circ_0001785低表达组39例与miR-330-5p高表达组45例、miR-330-5p低表达组35例,观察circ_0001785、miR-330-5p表达量与CRC患者临床病理参数的相关性;体外培养人CRC细胞SW480,分别将si-NC、si-circ_ 0001785、si-circ_ 0001785与anti-miR-NC、si-circ_ 0001785与anti-miR-330-5p转染至SW480细胞;采用qRT-PCR法检测细胞中circ_0001785、miR-330-5p的表达量;采用CCK-8法检测细胞增殖;Transwell小室实验检测细胞迁移及侵袭;双荧光素酶报告实验检测circ_ 0001785与miR-330-5p的靶向关系;Western blot法检测MMP-2、MMP-9蛋白表达量.结果 circ_0001785在CRC组织及细胞系中表达水平显著升高(P＜0.05),miR-330-5p的表达水平显著降低(P＜0.05);circ_0001785与miR-330-5p呈负相关(r=-0.985,P＜0.0001);circ_ 0001785、miR-330-5p表达量与分化程度、淋巴结转移及肿瘤分期密切相关(P＜0.05);与si-NC组比较,si-circ_0001785组细胞活力及MMP-2、MMP-9蛋白水平显著降低(P＜0.05),迁移及侵袭细胞数显著减少(P＜0.05);双荧光素酶报告实验证实circ_ 0001785可靶向结合miR-330-5p;下调miR-330-5p表达可降低干扰circ_0001785表达对CRC细胞SW480增殖、迁移及侵袭的作用.结论 circ_0001785在CRC中表达上调,而miR-330-5p表达下调,干扰circ_0001785表达可通过上调miR-330-5p的表达从而抑制CRC细胞增殖、迁移及侵袭.
- [1]蔡锐文,梁伟成,黄冀华,等.MicroRNA-29a在结直肠癌组织中的表达及意义[J].2019,(7).
- [2]陈晓燕,罗金键.CXCL8在结直肠癌组织中的表达及其临床意义[J].2018,(6).
- [3]张艳娜,孙江涛,刘孝民,等.沉默环状RNA hsa_circ_0001785表达对乳腺癌MDA-MB-231细胞增殖、迁移及侵袭能力的影响[J].2019,(2).
- [4]王金艳,张志勇,张学明,等.结直肠癌中miR-301b的表达及其临床意义[J].2019,(3).
- [5]Guangpu Yang,Tianhao Zhang,Jinning Ye,etc.Circ-ITGA7 sponges miR-3187-3p to upregulate ASXL1, suppressing colorectal cancer proliferation[J].2019,11.
- [6]Makoto, Inoue,Yohei, Uchida,Makoto, Edagawa,etc.The stress response gene ATF3 is a direct target of the Wnt/β-catenin pathway and inhibits the invasion and migration of HCT116 human colorectal cancer cells.[J].2018,13(7).
- [7]Shuai,Xiao,Mengyuan,Yang,Hao,Yang,etc.miR-330-5p targets SPRY2 to promote hepatocellular carcinoma progression via MAPK/ERK signaling.[J].2018,7(11).
- [8]Da-Chuang,Liu,Lin-Lin,Song,Qing,Liang,etc.Long noncoding RNA LEF1-AS1 silencing suppresses the initiation and development of prostate cancer by acting as a molecular sponge of miR-330-5p via LEF1 repression.[J].2019,234(8).
- [9]Yoo, Hye-In,Kim, Bong-Kyu,Yoon, Sungjoo Kim.MicroRNA-330-5p negatively regulates ITGA5 expression in human colorectal cancer[J].2016,36(5).
- [10]Zhenlong Chen,Rui Ren,Daiwei Wan,etc.Hsa_circ_101555 functions as a competing endogenous RNA of miR-597-5p to promote colorectal cancer progression[J].2019,38(32).
- [11]Yiting,Geng,Xiao,Zheng,Wenwei,Hu,etc.Hsa_circ_0009361 acts as the sponge of miR-582 to suppress colorectal cancer progression by regulating APC2 expression.[J].2019,133(10).
- [12]Di,Zhong,Peng,Li,Pei-Yao,Gong.Hsa_circ_0005075 promotes the proliferation and invasion of colorectal cancer cells.[J].2019,34(3).
- [13]Linsen, Feng,Jianhua, Ma,Haiming, Ji,etc.miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5.[J].2017,37(3).
- [14]Jiansheng,Chen,Taoliang,Chen,Yubo,Zhu,etc.circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma.[J].2019,38(1).
- [15]Zhang, Xiu-li,Xu, Ling-ling,Wang, Fang.Hsa_circ_0020397 regulates colorectal cancer cell viability, apoptosis and invasion by promoting the expression of the miR-138 targets TERT and PD-L1[J].2017,41(9).
- [16]Shenglong Li,Yi Pei,Wei Wang,etc.Circular RNA 0001785 regulates the pathogenesis of osteosarcoma as a ceRNA by sponging miR-1200 to upregulate HOXB2[J].2019,18(11).
- [17]Wei-Bing, Yin,Ming-Guang, Yan,Xiao, Fang,etc.Circulating circular RNA hsa_circ_0001785 acts as a diagnostic biomarker for breast cancer detection.[J].2017,(Spec ).
- [18]Tao,Shen,Xianshuo,Cheng,Xin,Liu,etc.Circ_0026344 restrains metastasis of human colorectal cancer cells via miR-183.[J].2019,47(1).