Analysis of KLF5 expression and its prognostic significance in gastric cancer based on Oncomine and Kaplan-Meier Plotter
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 12
- DOI:
- 10.11569/wcjd.v27.i12.734
- Key Word:
- 胃癌;KLF5基因;Oncomine数据库;预后
Abstract： 背景:胃癌(gastric cancer,GC)为临床上常见的消化系统肿瘤,确诊时大多为晚期患者预后不良,且临床上缺乏预后预测相关因子.目的:应用Oncomie和Kaplan-Meier Plotter数据库分析Kru-ppel样因子5(kruppel-like factor 5,KLF5)在GC中的表达及其与患者预后相关性.方法:收集Oncomie和Kaplan-Meier Plotter数据库中关于KLF5基因表达的相关数据集,深入挖掘KLF5基因在GC组织与正常胃组织中的差异表达情况,根据KLF5中位表达水平分为高表达组和低表达组,绘制生存曲线并用Log-rank检验比较KLF5高、低表达组患者总生存及无疾病进展生存是否存在差异.同时回顾性分析天津市宝坻区人民医院收治并手术治疗的41例GC患者的临床资料,采用免疫组化检测41例患者癌组织中KLF5表达情况,探讨KLF5表达与患者临床病理特征的关系.结果:Oncomine数据库共收录了424个关于KLF5因表达水平的相关研究,其中在癌组织vs正常组织中差异表达的有35个,与正常组织相比,8个数据集结果提示KLF5在癌组织中高表达,27个数据集在癌组织中低表达.聚类分析显示,KLF5与ST14,TMEM125等20个基因存在共表达情况(在GC中共同低表达或高表达),提示这些共表达基因在功能上可能存在相关性.Oncomine数据库中10个KLF5表达芯片数据集对比分析了GC组织与正常组织中KLF5基因的差异表达情况,其中有4个芯片数据提示了GC组织中KLF5表达水平明显升高.Kaplan-Meier Plotter数据库中,2个相关基因芯片数据分析了KLF5表达与GC患者预后的关系,Long-rank检验显示KLF5高表达患者总生存和无疾病进展生存均小于低表达组(P＜0.05).免疫组化分析显示41例GC患者中,KL F5阳性表达者29例,阳性率为70.7(29/41).KLF5阳性表达与患者临床病理特征如性别、年龄、肿瘤分期、分级等无明显相关性(P＞0.05).结论:KLF5在GC中高表达并与患者的不良预后有关,而KLF5表达与患者临床病理特征无关.
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