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Effect of H2S on gastric ischemia-reperfusion injury in rats
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 24
- DOI:
- 10.11569/wcjd.v26.i24.1448
- Key Word:
- H2S;氧化应激;胃缺血再灌注;胃黏膜;氧自由基
Abstract： 目的 研究H2S对大鼠胃缺血再灌注(gastric ischemiareperfusion,GI-R)引发氧化应激的影响及可能机制.方法 实验采用夹闭腹主动脉30 min再灌注1h的方法建立大鼠GI-R损伤模型.在此基础上,提前给予大鼠不同浓度NaHS腹腔注射14 d预处理后,Image J分析胃黏膜损伤大体面积,采用HE染色测定胃黏膜损伤深度.分别通过试剂盒和Western blot方法检测胃黏膜组织中氧化应激相关指标.结果 不同浓度的NaHS对GI-R后胃黏膜损伤的影响不同,其中10 μmol/L NaHS预处理尽管不影响大鼠血浆中H2S浓度,但显著减少GI-R诱发的胃黏膜面积和损伤深度.与GI-R组相比,NaHS预处理组中丙二醛,H2O2水平以及XOD,gp91phox,p67phox表达明显降低,同时,还原性谷胱甘肽水平和SOD活性显著增加.结论 外源性H2S通过减少GI-R引起的氧化应激,对胃黏膜起保护作用.
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