Abstract： AIM To explore the effect of live combined bifidobacterium,lactobacillus, and enterococcus powder plus lansoprazole on the clinical symptoms and mental health in patients with peptic ulcer. METHODS One hundred and eighteen patients with peptic ulcer were admitted to the Department of Gastroenterology, Tianjin Fifth Central Hospital from June 2017 to May 2018. The patients were randomly divided into an observation group and a control group. Both groups were treated with lansoprazole, and the observation group was additionally given live combined bifidobacterium, lactobacillus, and enterococcus powder. The clinical symptoms, the Symptom Checklist 90 (SCL-90) score, and the quality of life were compared between the two groups of patients before and after treatment. RESULTS The total score of SCL-90 and the scores of somatization, depression, anxiety, and hostility in patients with peptic ulcer were all higher than those of healthy ones (P ＜0.05). After treatment, the scores of abdominal pain, abdominal distention, sour regurgitation, and belching in the two groups were lower than those before treatment, and abdominal pain score was significantly lower in the observation group than in the control group (P ＜ 0.05). The scores of SCL-90 and somatization, depression, anxiety and hostility in both groups were significantly lower than those before treatment (P ＜ 0.05), but the difference between the control group and the observation group was statistically significant (P ＜ 0.05). After treatment, the quality of life scores in the two groups were significantly higher than those before treatment, and the quality of life scores in the observation group were significantly higher than those of the control group (P ＜ 0.05).CONCLUSION Live combined bifidobacterium, lactobacillus, and enterococcus powder plus lansoprazole can improve the clinical symptoms, mental health, and quality of life in patients with peptic ulcer.