Regulatory effect of emodin on NF-κB signaling pathway in rats with acute liver failure
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 9
- DOI:
- 10.11569/wcjd.v26.i9.543
- Key Word:
- 大黄素;急性肝衰竭;炎症;凋亡;NF-κB信号通路
Abstract： 目的 研究急性肝衰竭大鼠采用大黄素治疗后对核因子-κβ(nuclear factor-κB,NF-κB)信号通路的调控作用.方法 以D氨基半乳糖/脂多糖腹腔注射造模,治疗组和阳性对照组于造模前分别给予大黄素,联苯双酯,正常组以等量生理盐水腹腔注射,连续3d造模16h后,尾静脉取血处死.检测各组大鼠血清谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate transaminase,AST)、NO、白介素(interleukin,IL)-1β含量及肝组织中Caspase 3、Caspase 8活性;HE染色检测肝脏病理学形态;Western blot检测iNOS、环氧合酶-2(cyclooxygenase-2,COX-2)、Bcl-2相关x蛋白(Bcl-associated x protein,Bax)、B淋巴细胞瘤-2基因(B-cell lymphoma-2,Bcl-2)、增殖细胞核抗原(proliferation cell nucleus antigen,PCNA)、NF-κBp65及IκBα蛋白的表达.结果 正常组较模型组血清中ALT、AST含量明显升高(P＜0.01);大黄素治疗组可有效抑制ALT、AST含量的升高(P＜0.01).急性肝功能衰竭(acute liver failure,ALF)模型组血清中ALT、AST、NO、IL-1β含量及肝组织中Caspase 3、Caspase 8活性显著上升;iNOS、COX-2、Bax表达量升高,Bcl-2、PCNA表达量下降;NF-κB p65、IκBα磷酸化显著,大黄素治疗组可抑制此变化.结论 大黄素通过NF-κB信号通路,可降低ALF大鼠血清中肝功能酶和炎症因子含量,调节细胞凋亡相关蛋白表达,从而改善ALF大鼠症状.
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