Abstract： AIM To explore the clinical significance of dynamic electrocardiogram (ECG) heart rate variability in patients with peptic ulcer (PU) and the effect of systematic nursing intervention on dynamic ECG.METHODS One hundred and twenty-eight subjects were enrolled at the Second Hospital of Tianjin Medical University from July 2016 to July 2017,including 64 patients with PU (experimental group) and 64 healthy volunteers (control group).The control group was given conventional nursing,and the experimental group was given systematic nursing intervention.All subjects underwent dynamic ECG monitoring.The time domain index and frequency index of heart rate variability were statistically analyzed.The incidence of ECG artefacts and patient satisfaction were also analyzed.RESULTS The standard deviation of NN intervals,standard deviation of average NN intervals,and low frequency (LF) were lower in the experimental group than in the control group (124.5 ms ± 8.3 ms vs 130.3 ms ± 7.2 ms,109.2 ms ± 7.4 ms vs 128.4 ms ± 8.9 ms,122.4 ms/Hz ± 9.5 ms/Hz vs 136.3 ms ± 10.6 ms),but the differences were not significant (P ＞ 0.05).The root mean square of successive differences,the proportion derived by dividing NN50 by the total number of NN intervals,high frequency (HF),and LF/HF,which reflect changes in parasympathetic tone,differed significantly between the experimental group and the control group (45.8 ms ± 8.7 ms vs 25.8 ms ± 12.3 ms,13.5 ± 4.9 vs 10.9 ± 4.3,93.5 ms/Hz ± 8.3 ms/Hz vs 71.3 ms/Hz ± 9.5 ms/Hz,0.7 ± 1.4 vs 1.6 ± 3.1,P ＜ 0.05).The incidence of ECG artefacts was significantly lower in the experimental group than in the control group (P ＜ 0.05),while the rate of satisfaction to nursing was significantly higher in the experimental group (90.6％ vs 60.9％,P ＜ 0.05).CONCLUSION Systematic care intervention can reduce the incidence of ECG artefacts,improve patient satisfaction,and ensure the accuracy and effectiveness of dynamic ECG monitoring.