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Ursodeoxycholic acid alleviates Schistosoma japonicum induced liver fibrosis in mice

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Author:
No author available
Journal Title:
World Chinese Journal of Digestology
Issue:
28
DOI:
10.11569/wcjd.v25.28.2544
Key Word:
熊去氧胆酸;血吸虫肝纤维化;治疗作用;Ursodeoxycholic acid;Schistosomiasis liver fibrosis;Therapeutic effect

Abstract: AIM To evaluate the therapeutic effect of ursodeoxycholic acid (UDCA) on liver fibrosis induced by Schistosoma japonicum.METHODS Sixty-seven 6-8-week-old female C57BL/6J mice were randomly divided into a control group,an infection group,and three UDCA treatment groups (n =13 or 14 each).Liver fibrosis was induced in mice of all groups except the control group by abdominal infection with Schistosoma japonicum.UDCA at 50,90 and 140 mg/(kg·d) was intragstrically given to the three UDCA treatment groups.Eight weeks after treatment,serum aspartate transaminase (AST),alanine transaminase (ALT),hyaluronic acid (HA),and laminin (LN) were measured;collagen fiber was stained by Masson's trichromatic staining to detect the change in collagen fiber area;and the expression of type Ⅰ collagen (COL1) and a-smooth muscle actin (a-SMA) in liver tissues was detected by immunohistochemical method.RESULTS After UDCA treatment,serum levels of AST,ALT,HA,and LN were significantly reduced;liver fibrous septa became thinner or disappeared;and the number of diffuse cirrhosis nodules and the area of collagen became significantly lower (P < 0.05).Immunohistochemical staining showed that COL1 and α-SMA expression was significantly decreased (P < 0.05),in a concentration dependent manner.CONCLUSION UDCA alleviates schistosomiasis liver fibrosis,mainly by protecting the liver and reducing liver damage and extracellular matrix synthesis.

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