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Effect of Changweiqing on miR-30a/Beclin1 pathway in subcutaneous xenografts of human colon cell line HCT116/ L-OHP in nude mice

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Journal Title:
World Chinese Journal of Digestology
Key Word:
肠胃清;奥沙利铂;结肠癌;miR-30a;自噬;耐药;Changweiqing;Oxaliplatin;Colon cancer;miR-30a;Autophagy;Resistance

Abstract´╝Ü AIM To evaluate the correlation of miR-30a and autophagy gene Beclin1 with chemoresistance and to explore the possible mechanism for Changweiqing to reverse drug resistance in colon cancer therapy.METHODS A xenograft tumor model of oxaliplatin-resistant human colon cancer cell line HCT116/L-OHP was established in nude mice,and the mice were randomly divided into a control group,an L-OHP (oxaliplatin) group,a Changweiqing group,a low-dose Changweiqing + L-OHP group,and a high-dose Changweiqing + L-OHP group.The expression of miR-30a,Beclin1,and LC3 was evaluated by RT-PCR and immunohistochemistry,and cell apoptosis was evaluated by TUNEL assay.RESULTS The up-regulation of Beclin1 and LC3 expression,down-regulation of miR-30a expression,and decrease of apoptosis were observed in the L-OHP group.However,the down-regulation of Beclin1 and LC3 expression,up-regulation of miR-30a expression,and increase of apoptosis were observed in the Changweiqing plus L-OHP groups (P < 0.05 or P < 0.01).CONCLUSION L-OHP-induced protective autophagy to reduce apoptosis may be the mechanism of drug resistance.Changweiqing can reverse drug resistance,possibly by inhibiting autophagy and regulating the miR-30a/Beclin1 signal transduction pathway.

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