Expression of IL-1 and IL-10 mRNAs in colonic tissue of ulcerative colitis rats with abnormal sapra syndrome
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 9
- DOI:
- 10.11569/wcjd.v25.i9.775
- Key Word:
- 异常胆液质;溃疡性结肠炎;实时荧光定量逆转录聚合酶链反应;Abnormal sapra syndrome;Ulcerative colitis;Quantitative reverse transcription-polymerase chain reaction
Abstract： AIM To investigate the role of interleukin (IL)-1α,IL-1β and IL-10 in the pathogenesis and development of ulcerative colitis in rats with abnormal sapra syndrome.METHODS A rat model of abnormal sapra syndrome was used to develop UC using trinitro-benzene-sulfonic acid (TNBS)/ethanol.Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the differences in IL-1α,IL-1β and IL-10 mRNA expression levels in the colonic tissues between normal rats (control group) and UC rats with abnormal sapra syndrome (model group).RESULTS Rat signs,symptoms and colonic mucosa damage suggested that UC with abnormal sapra syndrome was successfully induced.qRT-PCR results showed that compared with the normal group,the expression of IL-1α,IL-1β and IL-10 mRNAs was significantly upregulated in the model group (P ＜ 0.05).CONCLUSIONImbalance of inflammatory factors may occur in colon tissue of UC rats with abnormal sapra syndrome.
- [1]王伟宁,张熙纯,刘丽.活动期溃疡性结肠炎发病机制的免疫学探讨[J].2003,(16).
- [2]口锁堂,吴焕淦,施达仁.白介素与溃疡性结肠炎[J].2006,(4).
- [3]李进,杨锐,邓豫,等.IL-1β、IL-10在大鼠溃疡性结肠炎模型中的表达[J].2010,(12).
- [4]Silvio Danese,Claudio Fiocchi.Etiopathogenesis of inflammatory bowel diseases[J].2006,(30).
- [5]Fiocchi Claudio.Future of IBD pathogenesis[J].2008.
- [6]F. OBERMEIER,N. DUNGER,U. G. STRAUCH,etc.Contrasting activity of cytosin-guanosin dinucleotide oligonucleotides in mice with experimental colitis[J].2003,134(2).
- [7]Sartor RB.Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis.[J].2006,3(7).
- [8]Bernard Khor,Agnès Gardet,Ramnik J. Xavier.Genetics and pathogenesis of inflammatory bowel disease[J].2011,474(Jun.16 TN.7351).
- [9]Myung-Han Hyun,Chung-Ho Lee,Min-Hyung Kang,etc.Interleukin-10 Promoter Gene Polymorphisms and Susceptibility to Asthma: A Meta-Analysis.[J].2013,8(1).
- [10]Livak KJ,Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.[J].2001,25(4).
- [11]Jian-Xin, Zhang,Sheng-Chun, Dang,Jian-Guo, Qu,etc.Changes of gastric and intestinal blood flow, serum phospholipase A2 and interleukin-1beta in rats with acute necrotizing pancreatitis.[J].2005,11.
- [12]A C, Anand,C M, Adya.Cytokines and inflammatory bowel disease.[J].1900,20(3).
- [13]C A, Dinarello.The IL-1 family and inflammatory diseases.[J].1900,20(5 Suppl 27).
- [14]Ye,D.-Q.,Li,R.,Pan,H.-F.,etc.Role of interleukin-10 and interleukin-10 receptor in systemic lupus erythematosus[J].2013,32(9).
- [15]Yang H,Rotter J I,Toyoda H,etc.Ulcerative colitis: a genetically heterogeneous disorder defined by genetic (HLA class II) and subclinical (antineutrophil cytoplasmic antibodies) markers.[J].1993.
- [16]Larry Borish.IL-10: Evolving concepts[J].1998,101(3).
- [17]Jasenka, Trifunovi,Larisa, Miller,eljko, Debeljak,etc.Pathologic patterns of interleukin 10 expression--a review.[J].2015,25(1).