Impact of An Soup of Miao nationality on expression of Occludin in intestinal mucosa and content of IL-22 in hepatic tissue in mice with acute liver failure
- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 1
- DOI:
- 10.11569/wcjd.v25.i1.49
- Key Word:
- 急性肝衰竭;紧密连接蛋白;白介素-22;Acute liver failure;Tight junction protein;Interleukin-22
Abstract： 目的 通过检测肠黏膜紧密连接蛋白Occludin的表达及肝组织中白介素-22(interleukin-22,IL-22)的含量,初步探讨腌汤对急性肝衰竭(acute liverfailure,ALF)的食疗作用.方法 40只♂SD大鼠随机分成正常组、模型组、双歧杆菌三联活菌组、腌汤腌组,每组10只,双歧杆菌三联活菌组、腌汤组分别予双歧杆菌三联活菌、腌汤灌胃,1次/d,连续9d,正常对照组、模型组则用等容积的生理盐水灌胃.于实验第8天,除正常组外,其余3组予硫代乙酰胺(生理盐水配制)按300 mg/kg皮下注射,1次/d,连续2d,建立大鼠ALF模型.于实验的第10天,股动脉放血处死大鼠,留取血清测血清谷丙转氨酶,谷草转氨酶含量;留取距回盲部3 cm回肠组织及肝组织行肠黏膜紧密连接蛋白Occludin及肝组织IL-22的检测.结果 与正常组比,各组大鼠血清转氨酶水平及肝组织IL-22含量明显增加(P＜0.01).与模型组比,双歧杆菌三联活菌组、腌汤组血清转氨酶的水平有所下降(P＜0.01),双歧杆菌三联活菌组与腌汤组相比,差别无统计学意义(P＞0.05);与模型组相比,双歧杆菌三联活菌组、腌汤组大鼠肝组织IL-22含量增加(P＜0.05),与双歧杆菌三联活菌组比较,腌汤组无明显差异(P＞0.05);除正常组外,各组大鼠肠黏膜Occludin表达水平下降(P＜0.01),与模型组相比,双歧杆菌三联活菌组、腌汤组肠黏膜Occludin的表达水平明显上升(P＜0.05),与双歧杆菌三联活菌组相比,腌汤组Occludin的表达水平差异无统计学意义(P＞0.05).结论 苗族腌汤可能通过保护大鼠肠黏膜屏障,上调IL-22的表达对ALF发挥食疗作用.
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