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Effects of pholidotaphenanthrene A on proliferation, apoptosis,migration, and cell cycle progression of hepatocellular carcinoma HCCLM3 cells
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- Author:
- No author available
- Journal Title:
- World Chinese Journal of Digestology
- Issue:
- 30
- DOI:
- 10.11569/wcjd.v24.i30.4177
- Key Word:
- PPTA;肝癌;HCCLM3细胞;增殖;周期;迁移;Pholidotaphenanthrene A;Hepatocellular carcinoma cells;HCCLM3 cells;Proliferation;Cell cycle;Migration
Abstract： 目的 初步研究PPTA对肝癌细胞HCCLM3增殖、周期和迁移的作用及机制.方法 分别采用四甲基偶氮唑蓝法(methyl thiazolyl tetrazolium,MTT)、流式细胞术、细胞划痕实验,观察PPTA对肝癌细胞HCCLM3增殖、周期和迁移的影响;采用细胞免疫荧光实验法观察PPTA对HCCLM3细胞肌动蛋白纤维的分布、丝状和片状伪足形成等的影响;采用Western blot法检测肝癌细胞HCCLM3中蛋白激酶B(protein kinase B,AKT)和细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)的表达.结果 PPTA对HCCLM3细胞有抑制作用,抑制HCCLM3细胞增殖的IC50在50 μmol/L以下.当PPTA浓度为40 μmol/L时,对HCCLM3细胞周期有抑制作用.PPTA对HCCLM3细胞划痕的相对愈合速度有抑制作用,随着浓度的升高抑制作用显著增强.细胞免疫荧光显示,HCCLM3细胞中丝状伪足增多,片状伪足减少,细胞排列更为紧密,当PPTA浓度为40 μmol/L,作用显著;Western blot显示,当PPTA浓度为20、40 μmol/L时,上调HCCLM3细胞的AKT表达,下调pAKT表达.结论 PPTA抑制肝癌细胞HCCLM3的增殖、周期、迁移,其作用机制可能是调控AKT和ERK相关的信号通路.
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