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shRNA-mediated silencing of hexokinase II inhibits proliferation but promotes apoptosis in SGC7901 cells
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 18
- DOI:
- 10.3969/j.issn.1009-3079.2010.18.003
- Key Word:
- 己糖激酶;短发夹状RNA;SGC7901;GES-1
Abstract： 目的:观察shRNA沉寂HK-Ⅱ基因的表达对胃癌细胞株SGC7901细胞增殖、凋亡的影响, 初步评价HK-Ⅱ基因治疗胃癌的应用前景.方法:取胃癌细胞株SGC7901及胃上皮细胞株GES-1细胞为研究对象, 将每株细胞分别分成5组(A: 干扰组; B: 阳性对照组; C: 阴性对照组; D: 脂质体组; E: 空白对照组). 用shRNA沉寂胃癌细胞株SGC7901及胃上皮细胞株GES-1细胞中HK-Ⅱ的表达. 用逆转录-聚合酶链反应(RT-PCR)检测转染shRNA后每株细胞中各组HK-Ⅱ mRNA的表达变化; 分别用MTT及流式细胞仪检测转染后细胞增殖、凋亡的变化.结果:HK-Ⅱ mRNA在胃癌细胞株SGC7901细胞中的表达明显高于胃上皮细胞株GES-1细胞(t = 12.119, P <0.01), HK-Ⅱ shRNA可以明显抑制两株细胞中HK-Ⅱ的表达(P <0.01).沉寂HK-Ⅱ的表达可抑制SGC7901细胞的增殖(F = 159.811, P <0.01), 并促进其凋亡(χ2= 21.324, P <0.01); 但对胃上皮细胞增殖(F =0.704, P = 0.592)及凋亡(χ2 = 1.007, P = 0.909)无明显影响.结论:沉寂HK-Ⅱ表达可以抑制胃癌细胞株SGC7901的增殖, 促进其凋亡; 但对胃上皮细胞株GES-1无明显影响. HK-Ⅱ可能成为胃癌治疗的一个靶点.
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