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Juglone inhibits the proliferation of human esophageal carcinoma EC1 cells
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1009-3079.2010.11.012
- Key Word:
- 肽酰脯氨酰顺反异构酶;Juglone;细胞凋亡;细胞周期
Abstract： 目的:测定Pin1抑制剂(Juglone)对食管癌细胞EC1生长增殖的影响,探讨Juglone的抗肿瘤作用.方法:体外培养人食管癌细胞系EC1,用MTT试验观察细胞生长增殖状况,流式细胞仪检测细胞周期以及细胞凋亡.结果:MTT试验表明,Juglone对EC1细胞生长有明显的抑制作用,且抑制作用随作用浓度和作用时间增加而增强.流式细胞仪检测表明,加入Juglone培养48 h后,EC1细胞出现G2期阻滞.Juglone药物(10、20、30 μmol/L)培养48 h后,EC1细胞的凋亡率明显增加,与对照组相比有统计学意义(9.06%,32.88%,53.18% vs 8.77%.均P<0.05).结论:Pin1抑制剂Juglone可以通过抑制Pin1表达从而抑制食管癌细胞的增殖,Pin1抑制剂有望成为新型的抗肿瘤治疗靶点.
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