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Ghrelin stimulates gastric acid secretion by rat gastric mucosal cells in vitro
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 34
- DOI:
- 10.3969/j.issn.1009-3079.2009.34.003
- Key Word:
- Ghrelin;胃黏膜上皮细胞;大鼠;胃蛋白酶;H+-K+-ATPase
Abstract： 目的:探讨Ghrelin 对胃外分泌功能的作用. 方法:从断奶大鼠中获得新鲜胃黏膜组织. 分离培养胃黏膜上皮细胞,培养30 h 后,实验组换为分别含有1×10-4 、1×10-3 、1×10-2 和1× 10-1 μmol/L Ghrelin 的新鲜培养液,对照组换为不含Ghrelin 的正常新鲜培养液. 继续培养4 h,收集培养液和细胞,分别测定细胞的相对活性(MTT 法)、培养液中胃蛋白酶活性、细胞中H+-K+-ATPase mRNA 表达和活性. 结果:实验组中细胞的相对活性与对照组相比没有发生明显的变化;1×10-3 μmol/L Ghrelin 可显著提高胃蛋白酶的活性(P<0.05). 1×10-3 μmol/L Ghrelin 可显著增加细胞中H+K+-ATPase mRNA 的表达(P <0.05),1×10-4 和1 ×10-3 μmol/L Ghrelin 明显增加了胃黏膜上皮细胞中H+-K+-ATPase 的活性(均P<0.05). 结论:Ghrelin 体外作用于胃黏膜上皮细胞可刺激胃蛋白酶和胃酸的分泌.
- [1]饶辉.影响断奶仔猪胃肠道pH值及胃蛋白酶活性的因素[J].2008,(3).
- [2]沈国顺,刘丽霞,刘春秀.早期断奶仔猪腹泻发生的原因及预防措施[J].2001,(6).
- [3]Schmidt PT,Levin F,Hellstrom PM,etc.Ghrelin stimulates motility in the small intestine of rats through intrinsic cholinergic neurons.[J].2004,121(1).
- [4]Sakata Ichiro,Matsubara Maki,Kangawa Kenji,etc.Ghrelin-producing cells exist as two types of cells, closed- and opened-type cells, in the rat gastrointestinal tract.[J].2002,23(3).
- [5]Natsui K,Hosoda H,Takaya K,etc.Stomach is a major source of circulating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in humans.[J].2001,86(10).
- [6]F, Hervatin,F, Benkouka,J C, Robert,etc.The ontogeny of rat gastric H+/K+-ATPase.[J].1989,985.
- [7]Kangawa K,Hosoda H,Chihara K,etc.The role of circulating ghrelin in growth hormone (GH) secretion in freely moving male rats.[J].2003,72(22).
- [8]Bouin M,Tomasetto C,Rio MC,etc.Ghrelin/motilin-related peptide is a potent prokinetic to reverse gastric postoperative ileus in rat.[J].2002,282(6).
- [9](德)B.施特尔马赫著 钱嘉渊译.酶的测定方法[M].1992.
- [10]M, Kojima,H, Hosoda,Y, Date,etc.Ghrelin is a growth-hormone-releasing acylated peptide from stomach.[J].1999,402(6762).
- [11]T L, Peeters.Central and peripheral mechanisms by which ghrelin regulates gut motility.[J].2003,54 Suppl 4.
- [12]Netti C,Sibilia V,Pagani F,etc.Evidence for a central inhibitory role of growth hormone secretagogues and ghrelin on gastric Acid secretion in conscious rats.[J].2002,75(2).
- [13]Lindstrom E,Norlen P,Dornonville de la Cour C,etc.Ghrelin stimulates gastric emptying but is without effect on acid secretion and gastric endocrine cells.[J].2004,120(1).
- [14]A, Terano,K J, Ivey,J, Stachura,etc.Cell culture of rat gastric fundic mucosa.[J].1982,83.
- [15]陈杰.家畜生理学[M].1900.