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SN-38-loaded polymeric micelles can inhibit the growth of human gastric adenocarcinoma BGC-823 cells
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 28
- DOI:
- No doi available
- Key Word:
- SN-38;纳米;胃癌;BGC-823;氧化应激
Abstract： 目的:检测喜树碱活性代谢产物SN-38载药纳米微球(SN-38-np)的各项特征,比较该栽药纳米微球与裸药抗人胃癌肿瘤细胞BGC-823的效果.方法:用溶剂分散法制备SN-38/PCL-PEG纳米微球.原子力显微镜和透射电子显微镜观察纳米微球形态,采用高效液相色谱法(HPLC)测定SN-38浓度并计算该栽药微球载药量、包封率及描绘其体外释放曲线.采用MTT法观察该微球对人胃癌细胞株BGC-823的生长抑制效果,荧光显微镜检测细胞内活性氧(ROS)水平.结果:微球为不规则的圆形,平均粒径小于100nm.载药量11%左右,包封率80%左右;SN-38纳米微球可稳定溶解于水中且具有良好的缓释特性:MTT结果显示,较低浓度的SN-38载药纳米微球在72 h抑制肿瘤效果明显优于SN-38裸药,同时计算IC50发现SN-38载药纳米微球在24 h和72 h的IC50明显低于SN-38裸药(P<0.05),两者48 h时间点的IC50相当;细胞内活性氧(ROS)检测结果显示:裸药和载药微球均可明显诱导ROS产生,在较低作用浓度时,SN-38载药纳米微球可比裸药诱导产生更多的细胞内ROS产物.结论:SN-38载药纳米微球可使细胞内达到并维持有效药物浓度,即使在较低作用浓度下亦可持续有效的抑制肿瘤细胞生长,效果明显优于相同浓度下SN-38裸药.
- [1]孙元珏,赵晖,郭跃武,等.草酸铂及羟基喜树碱联合亚叶酸钙和氟尿嘧啶治疗进展期结直肠癌的比较[J].2006,(19).
- [2]杨觅,丁义涛,黄兆琦,等.顺铂温敏载药粒子的制备表征[J].2007,(2).
- [3]Li Y,Wu XY,Taulier N,etc.Screening of lipid carriers and characterization of drug-polymer-lipid interactions for the rational design of polymer-lipid hybrid nanoparticles (PLN).[J].2006,23(8).
- [4]N, Kaneda,Y, Hosokawa,T, Yokokura,etc.Plasma pharmacokinetics of 7-ethyl-10-hydroxycamptothecin (SN-38) after intravenous administration of SN-38 and irinotecan (CPT-11) to rats.[J].1997,20.
- [5]Lu X,Ji C,Xu H.Resveratrol-loaded polymeric micelles protect cells from Abeta-induced oxidative stress.[J].2009,375(1/2).
- [6]Li X,Li R,Qian X,etc.Superior antitumor efficiency of cisplatin-loaded nanoparticles by intratumoral delivery with decreased tumor metabolism rate.[J].2008,70(3).
- [7]Qian X,Yang M,Yu L,etc.Enhanced efficiency of thermally targeted taxanes delivery in a human xenograft model of gastric cancer.[J].2008,97(8).
- [8]Ahmad I,Xuan T,Zhang JA.HPLC method for determination of SN-38 content and SN-38 entrapment efficiency in a novel liposome-based formulation, LE-SN38.[J].2006,41(2).
- [9]Ghobrial IM,Adjei AA,Witzig TE.Targeting apoptosis pathways in cancer therapy.[J].2005,55(3).
- [10]Donghui Zheng,Xiaolin Li,Huae Xu,etc.Study on docetaxel-loaded nanoparticles with high antitumor efficacy against malignant melanoma[J].2009,(7).
- [11]Lavergne O,Demarquay D,Bailly C,etc.Topoisomerase I-mediated antiproliferative activity of enantiomerically pure fluorinated homocamptothecins.[J].2000,43(11).
- [12]Romanowski M.,Williams J.,Lansdown R.,etc.Nanoparticle drug delivery system for intravenous delivery of topoisomerase inhibitors[J].2003,91(1).
- [13]Romberg B,Storm G,Hennink WE.Sheddable coatings for long-circulating nanoparticles.[J].2008,25(1).