You Position: Home > Paper
Dextran sulfate sodiuminduced low grade mucosal inflammation activates visceral hypersensitivity in rats
( views:396, downloads:72 )
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 16
- DOI:
- 10.3969/j.issn.1009-3079.2009.16.005
- Key Word:
- 肠易激综合征;黏膜低度炎症;内脏感觉过敏;腹壁退缩反射;腹壁肌电;葡聚糖硫酸钠
Abstract： 目的: 探讨结肠黏膜低度炎症(lowgrademucosal inflammation, LGMI)对大鼠内脏感觉的影响.方法: 健康♂SD大鼠40只, 随机分为LGMI组和对照组( n = 20). LGMI组大鼠给予15 g/L葡聚糖硫酸钠(DSS)饮用7 d, 然后饮用蒸馏水7 d, 对照组大鼠饮用蒸馏水. 第14天进行气囊扩张大鼠腹部回缩反射(AWR)评分和腹壁肌电测定; 实验结束后取结肠组织作常规病理学检查; 免疫组织化学染色观察腰膨大部脊髓c-Fos、P物质(SP)和降钙素基因相关肽(CGRP)表达变化.结果: LGMI组大鼠结肠黏膜炎症评分为1.56±0.78分, 对照组为0.46±0.54分, 2组比较差异有显著性意义( P = 0.003). 当结肠气囊压力为20 mmHg时, 2组大鼠的AWR评分和腹壁肌电幅值差异均无统计学意义; 当气囊压力为40、60、80 mmHg时, LGMI组的AWR评分和腹壁肌电幅值明显高于对照组(均P<0.05).LGMI组大鼠腰骶段脊髓c-Fos、SP、CGRP的平均吸光度值均高于对照组(165.26±10.12vs 126.52±11.48, 134.28±10.62 vs 120.82±8.92, 157.66±6.25 vs 118.67±5.68, 均P<0.01).结论: LGMI触发了内脏感觉过敏, 可能在IBS的发病中起重要作用.
- [1]张艳丽,王承党.葡聚糖硫酸钠结肠炎模型的制作方法、特点和影响因素[J].2006,(1).
- [2]Jun-Ho La,Tae-Sik Sung,Hyun-Ju Kim,etc.Peripheral corticotropin releasing hormone mediates post-inflammatory visceral hypersensitivity in rats[J].2008,(5).
- [3]Tae Woon Kim,Jae Nam Seo,Young Ho Suh,etc.Involvement of lymphocytes in dextran sulfate sodium-induced experimental colitis[J].2006,(2).
- [4]Jun-Ho La,Tae-Wan Kim,Tae-Sik Sung,etc.Visceral hypersensitivity and altered colonic motility after subsidence of inflammation in a rat model of colitis[J].2003,(12).
- [5]Fioramonti J,Bueno L.Effects of inflammatory mediators on gut sensitivity.[J].1999,13(Suppl.A).
- [6]Bennis M,Rampin O,Ghanima A.c-Fos expression as endogenous marker of lumbosacral spinal neuron activity in response to vaginocervical-stimulation.[J].2002,9(1).
- [7]Morilak DA,Gerhardt GA,Gould GG,etc.Effects of chronic antidepressant treatments on serotonin transporter function, density, and mRNA level.[J].1999,19(23).
- [8]Spiller RC.Role of infection in irritable bowel syndrome.[J].2007,42(Suppl.17).
- [9]Ohman L,Lundgren A,Simren M,etc.A controlled study of colonic immune activity and beta7+ blood T lymphocytes in patients with irritable bowel syndrome.[J].2005,3(10).
- [10]Chevalier E,Friese N,Dahl SG,etc.Involvement of prostaglandins and CGRP-dependent sensory afferents in peritoneal irritation-induced visceral pain.[J].1997,70(1).
- [11]Jennifer RO’,Hara,Alan ELomax,etc.Ileitis alters neuronal and enteroendocrine signalling in guinea pig distal colon[J].2007,56(2).
- [12]Andrew W, Dupont.Post-infectious irritable bowel syndrome.[J].2007,9(5).
- [13]SM Collins,K McHugh,K Jacobson,etc.Previous inflammation alters the response of the rat colon to stress[J].1996,111(6).
- [14]周吕 柯美云.神经胃肠病学与动力-基础与临床[M].2005.
- [15]Ferraz JG,Beck P,Bunnett NW,etc.Role for protease activity in visceral pain in irritable bowel syndrome.[J].2007,117(3).
- [16]Greenwood Van Meerveld B,Mawe GM,Coates MD,etc.Effects of serotonin transporter inhibition on gastrointestinal motility and colonic sensitivity in the mouse.[J].2006,18(6).
- [17]L Bueno,J Fioramonti,M Delvaux,etc.Mediators and pharmacology of visceral sensitivity: From basic to clinical investigations[J].1997,112(5).