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Effects of octreotide on expression of Fas, FasL and P53 in human gastric cancer SGC-7901 cells
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 7
- DOI:
- 10.3969/j.issn.1009-3079.2009.07.012
- Key Word:
- 胃肿瘤;凋亡;奥曲肽;Fas;FasL;P53;逆转录多聚酶链反应;流式细胞术
Abstract： 目的:探讨奥曲肽(OCT)对胃癌细胞SGC-7901中Fas,FasL,P53,P21,P27和C-Myc表达的影响.方法:流式细胞术和RT-PCR法分别用于检测经OCT(1×10-7 mol/L)处理前后胃癌细胞SGC-7901中Fas,FasL,P53,P21,P27,c-Myc阳性癌百分率、mRNA表达和蛋白表达及细胞周期G0/G1、S、G2/M的变化.结果:胃癌细胞SGC-7901经OCT诱导6、12、24和48 h垢,Fas和FasL mRNA表达均明显增加,P53 mRNA表达则显著减少,而P21,P27和c-Myc mRNA表达均无明显变化;胃癌细胞SGC-7901经OCT诱导12 h后,Fas和FasL蛋白相对表达强度也显著增加,P53蛋白相对表达强度则明显降低(5.5±0.3 vs 3.2±0.1,5.1±0.3 vs 4.5±0.1.3.3±0.2 vs 4.9±0.3,P＜0.05或0.01),而P21,P27和c-Myc蛋白相对表达强度均无明显改变.细胞周期G0/G1、S、G2/M在OCT处理前后的变化并不明显.结论:OCT能上调SGC-7901胃癌细胞中Fas和FasL表达,下调突变型p53表达,是其诱导凋亡作用的重要机制之一.
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