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Expression of apoptosis-related Bcl-2-family and P53 in human adenocarcinoma of the pancreas
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 4
- DOI:
- 10.3969/j.issn.1009-3079.2009.04.006
- Key Word:
- 胰腺肿瘤;P53;Bcl-2;Bax;Bcl-xL;BclxS;蛋白免疫印迹法;原位缺口末端标记法
Abstract： 目的:探讨胰腺癌组织(pancreatic carcinoma,PC)中P53和Bcl-2家族(Bcl-2,Bax,Bcl-xL,BclxS)蛋白表达及与细胞凋亡的关系.方法:免疫组化法检测35例PC中P53蛋白表达,将PC分为P53阴性表达组(第1组,n = 18)和P53阳性表达组(第2组,n = 17);Western blot检测两组PC中P53,Bcl-2,Bax,Bcl-xL和BclxS蛋白表达,TUNEL法检测第1组中凋亡指数(AI).结果:Bcl-2蛋白在P53阳性PC表达上调,而在P53阴性PC表达下调( P = 0.047);Bax和Bcl-xL蛋白在两组PC中表达都明显上调( P = 0.274,0.334);Bcl-xS在P53阳性表达PC明显下调,在P53阴性表达组明显上调( P = 0.01);在P53阴性和阳性表达PC,AI分别为12.1±2.47和9.1±1.48( P = 0.023);Bcl-2家族各成员表达与AI无相关性,而Bcl-2/Bax比率与AI有明显的相关性( P<0.01).结论:Bcl-2是依赖P53调节的抗凋亡蛋白,BclxS是依赖P53调节的促凋亡蛋白,而P53主要通过调节Bcl-2/Bax比率发挥凋亡调节作用.
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