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Influence of liposomal clodronate upon peritoneal macrophages in rats

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
4
DOI:
10.3969/j.issn.1009-3079.2009.04.002
Key Word:
氯膦酸二钠脂质体;腹腔巨噬细胞;NO;O2-;H2O2

Abstract´╝Ü 目的:探讨氯膦酸二钠脂质体(liposomal clodronate,LC)的制备及对大鼠腹腔巨噬细胞(peritoneal macrophages,PM)功能的影响.方法:利用薄膜法制备LC;分离培养大鼠PM,用不同浓度的LC(50,100,150 μmol/L)分别作用于大鼠PM,然后采用MTT比色法,中性红比色法,检测NO,O2-和H2O2的含量来评估巨噬细胞功能.结果:在三种不同浓度LC的干预下,MTT结果显示巨噬细胞的生长受到明显抑制( P<0.01);巨噬细胞吞噬中性红结果表明其吞噬功能受到明显抑制,抑制率分别为17.4%,25.8%和38.0%;巨噬细胞分泌NO、O2-的能力也受到明显抑制,与对照组相比差异具有显著性(48.398±0.667,27.664±0.566,25.626±0.184vs 91.714±1.726;0.289±0.017,0.256±0.013,0.216±0.003 vs 0.342±0.022,均P<0.05);巨噬细胞产生H2O2的能力仅在100 μmol/L与150μmol/L时受到明显抑制(0.261±0.002,0.213±0.015 vs 0.277±0.003,均P<0.05).结论:LC对大鼠PM功能具有抑制作用.

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