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Correlation analysis among RUNX3, VEGF and MVD and their significance using gastric cancer tissue microarray
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 3
- DOI:
- 10.3969/j.issn.1009-3079.2009.03.019
- Key Word:
- 胃癌;组织芯片;人类相关转录因子3;血管内皮生长因子;微血管密度
Abstract： 目的: 探讨胃癌组织芯片中RUNX3、VEGF表达与MVD的相关性及其意义.方法: 采用组织芯片技术建立经确证8 0例胃癌的组织芯片, 免疫组化检测芯片中RUNX3、VEGF的表达及微血管密度计数.结果: RUNX3在胃癌芯片中表达阳性率分别为阴性46.3%(37/80)、弱阳性35.0%(28/80)和强阳性18.7%(15/80); VEGF的表达阳性率分别为阴性22.5%(18/80)、弱阳性30.0%(24/80)和强阳性47.5%(38/80), 其阳性表达部位为细胞质. MVD值RUNX3表达阳性者显著低于表达阴性者; VEGF表达阳性者显著高于表达阴性者, RUNX3的表达和MVD之间存在显著负相关( rs = -0.742, P<0.01), VEGF的表达和MVD之间存在显著正相关( rs = 0.683, P<0.01).VEGF表达阳性率在RUNX3阴性组逐渐增高;在RUNX3弱阳性组逐渐增高, 在RUNX3强阳性组逐渐降低, RUNX3的表达和VEGF之间存在显著的负相关( rs = -0.333, P<0.01).结论: RUNX3作为一种抑癌基因可能通过某种机制抑制VEGF的表达减少胃癌的血管新生、生长和转移; 联合检测RUNX3、VEGF和MVD的表达, 对把握胃癌生物学行为和预后有一定的价值.
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