You Position: Home > Paper
Detection of peripheral blood human telomerase reverse transcriptase mRNA in colorectal cancer with real-time fluorescent quantitative RT-PCR and its clinical Significance
( views:374, downloads:25 )
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 36
- DOI:
- 10.3969/j.issn.1009-3079.2008.36.008
- Key Word:
- 结直肠癌;微转移;外周血端粒酶逆转录酶(hTERT)mRNA;实时荧光定量逆转录聚合酶锚反应
Abstract： 目的:建立外周血端粒酶逆转录酶(hTERT)mRNA表达量检测的实时荧光定量逆转录聚合酶链反应(RT-PCR)系统,检测结直肠癌患者hTERT mRNA的表达并探讨其对结直肠癌微转移的诊断价值.方法:用实时荧光定量RT-PCR技术检测53例结直肠癌患者和21名健康人的外周血标本hTERT mRNA表达情况,分析其表达与肿瘤临床病理特征的关系并应用接受者操作特征曲线(ROC曲线)分析hTERT mRNA检测对结直肠癌的诊断价值.结果:结直肠癌组外周血hTERT mRNA表达水平显著高于正常对照组(t1=7.953,P＜0.05).其表达与肿瘤淋巴结转移、血行转移和TNM分期相关(t'/t=2.334,2.149,2.460,均P＜0.05)hTERT mRNA诊断结直肠癌的ROC曲线下面积0.91,诊断界值为Ct≤32.结论:应用实时荧光定量RT-PCR技术克服了传统PCR只能定性检测而不能定量检测的缺-点:外周血hTERT mRNA可作为诊断结直肠癌微转移的标记物.
- [1]Xi-wei Zhang,Hong-Yu Yang,PING Fan,etc.Detection of micrometastasis in peripheral blood by multi-sampling in patients with colorectal cancer[J].2005,(3).
- [2]亓玉琴,刘吉勇,朱菊人.端粒酶在大肠活检组织中的表达及其临床意义[J].2000,(3).
- [3]Dasi F,Ripoll R,Garcia-Conde J,etc.Real-time quantification in plasma of human telomerase reverse transcriptase (hTERT) mRNA: a simple blood test to monitor disease in cancer patients.[J].2001,81(5).
- [4]Shiota G,Sano A,Hasegawa J,etc.Sensitive detection of human telomerase reverse transcriptase mRNA in the serum of patients with hepatocellular carcinoma.[J].2003,64(4).
- [5]Atkinson SP,Przyborski S,Stewart R,etc.A key role for telomerase reverse transcriptase unit in modulating human embryonic stem cell proliferation, cell cycle dynamics, and in vitro differentiation.[J].2008,26(4).
- [6]主编余松林.医学统计学[M].2002.
- [7]J.A. MARCH,P. MARTINEZ-RODES,M. GIL,etc.Real-Time Quantification of Human Telomerase Reverse Transcriptase mRNA in the Plasma of Patients with Prostate Cancer[J].2006,1075(sep).
- [8]Lledo SM,Ripoli R,Cervantes A,etc.Real time quantification in plasma of human telomerase reverse transcriptase (hTERT) mRNA in patients with colorectal cancer.[J].2004,6(4).
- [9]Nwokolo CU,Barclay JY,Morris A.Telomerase, hTERT and splice variants in Barrett's oesophagus and oesophageal adenocarcinoma.[J].2005,17(2).