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Effect of expression silencing of NF-KB in RAW264.7macrophages on secretory cytokines in systemic inflammatory response syndrome

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Author:
No author available
Journal Title:
WORLD CHINESE JOURNAL OF DIGESTOLOGY
Issue:
36
DOI:
10.3969/j.issn.1009-3079.2008.36.006
Key Word:
基因沉默;RNA干扰;核因子-kB;IκB激酶;脂多糖;诱生性-化氮合酶;肿瘤坏死因子-a;白介素-10;巨噬细胞

Abstract´╝Ü 目的:探讨IκB蛋白激酶α及γ基因沉默对巨噬细胞分泌的NF-kB依赖性的下游炎性因子表达的影响.方法:应用RNA干扰技术将IκB蛋白激酶仅α及γ基因沉默后,观察RAW264.7小鼠巨噬细胞经LPS刺激后,NF-kB的活化以及多种NF-kB依赖性的下游炎性因子的表达.结果:RNA干扰处理后RAW264.7巨噬细胞中IKKα及SIKKγ基因表达出现明显下调,而对照组中IKKαy及IKKγ基因表达无明显变化.经LPS刺激活化,IKKα和IKKγ表达随作用时间逐渐增强.IKKα及IKKγ基因沉默后,可导致TNF-α、iNOS、IL-10、IKBα等多种炎症相关基因表达的明显下调.结论:IKKα及IKKγ两种蛋白激酶参与NF-kB信号通路的调节,NF-kB信号通路的活化是全身性炎症反应的中心环节.

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