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Effect of vascular endothelial growth factor 165 on the invasion and metastasis of human gastric cancer cells in vitro
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 36
- DOI:
- 10.3969/j.issn.1009-3079.2008.36.002
- Key Word:
- 血管内皮生长因子165;胃癌;迁移;转移
Abstract： 目的:探讨血管内皮生长因子165基因(vascular endothelial growth factor 165,VEGF165)对胃癌侵袭转移的影响及可能的机制.方法:通过体外培养人胃癌细胞BGC-823,分别转染Ad-GFP及Ad-VEGF165*应用Millicell小室分析VEGF165转染前后胃癌细胞迁移能力的变化:逆转录聚合酶链反应(RT-PCR)研究VEGF懈转染前后BGC-823细胞MMP-2、u-PAmRNA表达变化.结果:迁移实验结果显示Ad-VEGF15组胃癌细胞迁移能力高于Ad-GFP组及对照组(217.53±15.77vs174.07±13.83,167.5±11.5,P＜0.05);RT-PCR结果显示VEGF懈转染后促进了BGC-823细胞MMP-2、u-PA的mRNA表达,Ad-VEGF165组MMP-2、u-PA的mRNA水平明显高于对照组及d-GFP组((MMP-2mRNA:0.6646±0.0486 vs 0.3803±0.0254.0.4096±0.0668;u-PA mRNA:0.8216±0.0798vs0.4043±0.0620,0.406±0.1158,均P＜0.05).结论:经VEGF165转染后,胃癌细胞迁移能力增强,MMP-2、u-PA的mRNA表达增加,MMP-2、u-PA表达的增加可能为VEGF促进胃癌侵袭转移机制.
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