Effects of sinomenine on matrix metalloproteinases of trinitrobenzene sulfonic acid-induced colitis in rats
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 30
- DOI:
- 10.3969/j.issn.1009-3079.2008.30.005
- Key Word:
- 结肠炎;肿瘤坏死因子-α;基质金属蛋白激酶;青藤碱
Abstract： 目的:研究青藤碱对三硝基苯磺酸诱导的实验性结肠炎的防治作用.方法:三硝基苯磺酸/乙醇制备大鼠结肠炎模型,40只SD大鼠随机均分为正常组、模型组、5-ASA组及青藤碱治疗组.正常组与模型组每天每只给予生理盐水2 mL,5-ASA组给予5-ASA 100 mg/(kg·d),青藤碱组给予青藤碱120 mg/(kg·d),连续灌胃14 d.评价各组大鼠结肠大体形态损伤指数(CMDI)和组织学损伤指数(TDI),ELISA法检测血清TNF-α、MMP-2和MMP-9含量,免疫组织化学法检测结肠组织MMP-2和MMP-9的表达.结果:模型组大鼠CMDI、TDI,血清TNF-α、MMP-2和MMP-9含量,结肠组织MMP-2和MMP-9表达均显著高于正常组(均P<0.01),5-ASA和青藤碱能明显减弱CMDI和TDI(1.30±1.16,1.00±0.82 vs 6.50±1.27;1.10±1.20,0.90±0.99 vs 5.10±0.74,均P<0.01),降低血清TNF-α、MMP-2和MMP-9含量及结肠组织MMP-2和MMP-9的表达(35.37±4.44,32.50±4.91 vs 74.72±5.30;2.44±0.83,2.50±0.82 vs 10.40±2.10;5.88±1.84,5.32±1.81 vs15.85±2.39;1.30±1.25,1.00±0.94 vs 5.00±1.41;1.50±1.35,1.40±0.97 vs 6.30±0.67;均P<0.01),5-ASA组与青藤碱组差异无显著统计学意义.结论:青藤碱能明显降低结肠炎大鼠TNF-α、MMP-2和MMP-9的表达,对大鼠结肠炎发挥良好的抗炎作用.
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