Effect of Qinggan Huoxue recipe and its separated recipes on the expression of CD14 and TLR4 in rats with alcoholic liver disease
- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 30
- DOI:
- 10.3969/j.issn.1009-3079.2008.30.003
- Key Word:
- 清肝活血方;清肝方;活血方;酒精性肝病;CD14;TLR4
Abstract： 目的:探讨清肝活血方及其拆方对酒精性肝病(alcoholic liver disease,ALD)大鼠肝组织CD14、TLR4表达的影响.方法:♂ Wistar大鼠100只随机分成空白组、CCl4组各10只,余80只为造模组,采用复合因素复制ALD模型6 wk.CCl4组予微量CCl4腹腔注射,每周2次.造模4 wk后将模型组随机分成4组,清肝活血方及其拆方组各15只,余为模型组.予等效剂量清肝活血方及拆方ig 2 wk.检测血清ALT,AST水平:留取肝脏标本进行HE染色.RT-PCR检测肝组织CD14 mRNA、TLR4 mRNA表达,免疫组织化学染色检测肝组织CD14表达,Wlestern blot检测肝组织TLR4蛋白表达.结果:与模型组比较,清肝活血方及其拆方均可明显改善ALD大鼠肝脏脂肪变及肝脏炎症程度(0.67±0.50,2.15±1.28,1.38±1.06 vs4.56±0.73,均P<0.01).清肝活血方能显著降低模型大鼠血清ALT水平(725.65±111.02 vs884.68±177.54,P<0.05),清肝方和活血方无明显作用;清肝方、活血方、清肝活血方均可明显降低ALD大鼠血清AST水平(2383.81±888.18,2158.93±922.85,2001.90±519.27vs 3210.98±640.63.P<0.01或0.05),组间比较无显著差异.清肝方及清肝活血方能显著降低模型大鼠CD14 mRNA表达(1.46±0.52,1.10±0.40 vs 2.67±0.66,均P<0.01).活血方作用不明显,清肝活血方优于活血方.清肝方、活血方、清肝活血方均能显著降低模型大鼠肝组织TLR4 mRNA表达(1.91±0.03,2.11±0.03,1.53±0.01 vs 2.37±0.03,均P<0.01),组间比较无显著差异.清肝活血方显著降低模型大鼠肝组织CD14表达(13 392.28±9287.54vs 32 288.89±15 631.03,P<0.01).清肝方、活血方、清肝活血方均能显著降低模型大鼠肝组织TLR4表达(1.06±0.10,1.19±0.05,0.98±0.12 vs 1.40±0.11,均P<0.01).结论:清肝活血方及其拆方发挥对ALD的防治作用,其作用机制可能与降低CD14、TLR4基因和蛋白的表达有关.
- [1]王磊,季光,郑培永,等.大鼠酒精性肝纤维化复合模型的建立[J].2006,4(3).
- [2]许亦斌,季光,曹承楼,等.“清肝活血方”对肝星状细胞增殖及胶原生成的影响[J].2003,(3).
- [3]季光,王淼,郑培永,等.清肝活血方及其拆方对库普弗细胞表达CD14、Toll样受体4及核因子-κB的影响[J].2006,4(5).
- [4]王淼,龙爱华,刘成海,等.清肝活血方及其拆方对脂多糖介导库普弗细胞活化ERK表达调控的研究[J].2007,(5).
- [5]华静,邱德凯,李继强,等.内毒素受体CD14在四氯化碳诱导的大鼠肝损伤和纤维化过程中的变化[J].2006,(1).
- [6]季光.清肝活血方治疗酒精性肝病的临床研究[J].2003,1(2).
- [7]吴涛,季光,郑培永.CD14与酒精性肝病[J].2006,(35).
- [8]季光,王淼,龙爱华,等.清肝活血方和拆方对脂多糖介导库普弗细胞活化的调控作用研究[J].2007,(1).
- [9]季光,曹承楼,张广业,等.清肝活血方对酒精性肝病大鼠肝组织Ⅰ型胶原表达的影响[J].2002,(1).
- [10]Andonegui G,Kubes P,Goyert SM.Lipopolysaccharide-induced leukocyte-endothelial cell interactions: a role for CD14 versus toll-like receptor 4 within microvessels.[J].2002,169(4).
- [11]王泰龄,刘霞,周元平.慢性肝炎炎症活动度及纤维化程度计分方案[J].1998,(4).
- [12]R G, Knodell,K G, Ishak,W C, Black,etc.Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis.[J].1900,1.
- [13]Takehiko Uesugi,Michael D.Wheeler,Ronald G.Thurman,etc.Reduced Early Alcohol-Induced Liver Injury in CD14-Deficient Mice[J].2001,166(7).
- [14]Alain Haziot,Enza Ferrero,Frank Köntgen,etc.Resistance to Endotoxin Shock and Reduced Dissemination of Gram-Negative Bacteria in CD14-Deficient Mice[J].1996,4(4).
- [15]K M, Ryan,M K, Ernst,N R, Rice,etc.Role of NF-kappaB in p53-mediated programmed cell death.[J].2000,404(6780).
- [16]Kalousova M,Zima T.Oxidative stress and signal transduction pathways in alcoholic liver disease.[J].2005,29(11 Suppl.).
- [17]Michael D, Wheeler.Endotoxin and Kupffer cell activation in alcoholic liver disease.[J].2003,27(4).
- [18]Diehl A.M.,Goodman Z.,Ishak K.G..Alcohollike liver disease in nonalcoholics[J].1988.
- [19]J D, Lee,K, Kato,P S, Tobias,etc.Transfection of CD14 into 70Z/3 cells dramatically enhances the sensitivity to complexes of lipopolysaccharide (LPS) and LPS binding protein.[J].1992,175.
- [20]Reuben A.Alcohol and the liver.[J].2008,24(3).