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Effects of leptin on the content of triglyceride and the expression of peroxisome proliferator activated receptor α and carnitine palmitoyltransferase-I in human hepatocytes with fatty degeneration
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 25
- DOI:
- 10.3969/j.issn.1009-3079.2008.25.004
- Key Word:
- 瘦素;人肝L-02细胞;过氧化物酶体增殖物激活受体α;肉碱转移酶-I;高效液相色谱法;逆转录-聚合酶链反应
Abstract： 目的:探讨瘦素对肝细胞脂质变性的作用及其机制.方法:以离体人肝L-02细胞株制备肝细胞脂肪变性模型.实验分为正常肝细胞组、人肝L-02细胞脂肪变性模型组、瘦素不同浓度处理Ⅰ、Ⅱ、Ⅲ组(浓度分别为10-8、10-7、10-6mol/L)和阳性对照组.孵育24 h后,油红O染色观察细胞形态和细胞内脂滴的形成,高效液相色谱法(HPLC)检测细胞内甘油三酯(TG)的含量.RT-PCR检测过氧化物酶体增殖物激活受体α(PPARα)及其目标基因肉碱转移酶-I(CPT-D) mRNA水平的表达.结果:模型组和瘦素I组细胞质中有较多的脂滴形成,而瘦素Ⅱ、Ⅲ组、正常组、阳性对照组胞质中脂滴较少.瘦素浓度为10-8、10-7、10-6mol/L时,肝细胞中TG的含量为1.063±0.146、0.648±0.023、0.553±0.045 mmoL/g蛋白,呈细胞依赖性减少.瘦素Ⅱ、Ⅲ组与阳性对照组比较.PPARα仅的mRNA表达上升更为明显,差别有统计学意义(p<0.01);但瘦素I组与阳性对照组之间无显著性差异.模型组与正常组比较,CPT-I mRNA表达无明显改变,经瘦素处理后,较模型组相比,CPT-I mRNA表达明显上升(p<0.01).结论:瘦素能降低人肝L-02脂变细胞内TG的含量,呈剂量依赖关系,其降低细胞内TG作用可能与PPARα及其目标基因表达上调有关.
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