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Effects of N-acetylcysteine on the detoxification and biological metabolism of C3A immortalized cell line
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- Author:
- No author available
- Journal Title:
- WORLD CHINESE JOURNAL OF DIGESTOLOGY
- Issue:
- 23
- DOI:
- 10.3969/j.issn.1009-3079.2008.23.009
- Key Word:
- C3A细胞;N-乙酰半胱氨酸;慢性重型肝炎;解毒;代谢
Abstract： 目的:研究N-乙酰半胱氨酸(N-acetylcysteine,NAC)对慢性重型肝炎肝衰竭时肝细胞解毒代谢功能的影响,并比较NAC与还原型谷胱甘肽(GSH)的疗效.方法:体外慢性重型肝炎患者血浆(chronic severe hepatitis plasma,CSHP)培养C3A细胞,并同时分别加入大、中、小三个剂量NAC及对应剂量的GSH,以正常血浆(normal human plasma,NHP)及培养基(100 mL/L FBS-MEM)为对照,分别于24、48、72h 3个时间点检测细胞内GSH及脂质过氧化物(MDA)的含量,并检测安定代谢量的变化.结果:24、48、72h 3个时间点.培养基组、正常血浆组、各剂量NAC治疗组和GSH治疗组C3A细胞中GSH含量与安定代谢量均分别高于慢性重型肝炎血浆组(GSH:F=246.116,235.489,201.536,均P＜0.01;安定代谢量:F=306.812,476.722,502.061,均P＜0.01),而慢性重型肝炎血浆组C3A细胞中MDA含量均分别高于上述各组(F=332.48,662.349,492.983,均P＜0.01).慢性重型肝炎血浆+NAC组与慢性重型肝炎血浆+GSH组比较,前者细胞内的GSH含量和对安定的代谢量,大、中、小剂量组均分别高于后者对应剂量组(P＜0.01),而前者细胞内的MDA含量,大、中、小剂量组均分别低于后者对应剂量组(P＜0.01).结论:NAC可以显著改善慢性重型肝炎肝衰竭时肝细胞解毒代谢功能,GSH也有相似功效,且相同摩尔剂量的NAC效果要优于GSH.
- [1].病毒性肝炎防治方案[J].2001,(1).
- [2]郑英,楼宜嘉.微粒体谷胱甘肽S-转移酶与药物代谢[J].2003,(7).
- [3]邹正升,陈菊梅,辛绍杰,等.565例重型病毒性肝炎的临床特点分析[J].2001,(4).
- [4]中华医学会感染病学分会肝衰竭与人工肝学组,中华医学会肝病学分会重型肝病与人工肝学组.肝衰竭诊疗指南[J].2006,(9).
- [5]Aninat C,Descheemaeker PN,Guillouzo A,etc.Catecholamines induce an inflammatory response in human hepatocytes.[J].2008,36(3).
- [6]Keatch SA,Rangar D,Plevris JN,etc.Improvement of C3A cell metabolism for usage in bioartificial liver support systems.[J].2004,41(4).
- [7]Lim Y,Levy MA,Bray TM.Dietary supplementation of N-acetylcysteine enhances early inflammatory responses during cutaneous wound healing in protein malnourished mice[J].2006,17(5).
- [8]Estabrook RW.A PASSION FOR P450s (REMEMBRANCES OF THE EARLY HISTORY OF RESEARCH ON CYTOCHROME P450).[J].2003,31(12).
- [9]Bennett BrianM,Toader Violeta,Ji Yanbin.Regulation of microsomal and cytosolic glutathione S-transferase activities by S-nitrosylation.[J].2002,63(8).
- [10]Hirsh M,Manov I,Iancu TC.Acetaminophen hepatotoxicity and mechanisms of its protection by N-acetylcysteine: a study of Hep3B cells.[J].2002,53(6).
- [11]A D, Smirthwaite,J D, Gaylor,R B, Cousins,etc.Cytotoxicity of bile in human Hep G2 cells and in primary cultures of rat hepatocytes.[J].1998,22(10).