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Phosphoglycerate kinase 1 as a candidate of tumor-associated antigen identified from esophageal squamous cell carcinoma

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Author:: No author available

Journal Title:: WORLD CHINESE JOURNAL OF DIGESTOLOGY

Issue:: 17

DOI:: 10.3969/j.issn.1009-3079.2008.17.007

Key Word:: 食管鳞癌;肿瘤相关抗原;磷酸甘油酸激酶

Abstract：目的:鉴定新的食管鳞癌肿瘤相关抗原.方法:食管癌EC0156细胞总蛋白先经亚组分预分离,有效富集胞质、胞膜和胞核等组分蛋白;胞质组分蛋白经SDS-PAGE分离后分别与食管癌患者血清或健康志愿者血清共孵育,分离血清结合蛋白条带;胶内酶解阳性蛋白条带,肽段经色谱分离后用SynaptTM HDMS质谱鉴定.候选蛋白进一步Western blot经免疫组化验证.结果:总蛋白经亚组分分离后,不同组分蛋白均得到有效富集.食管癌患者血清能与EC0156细胞胞质蛋白结合,即选择性识别肿瘤相关抗原.其中,43kDa蛋白条带与食管癌血清(41.4%,12/29)和对照(3.6%,1/28)结合阳性率具有明显差异.从该蛋白条带中共鉴定到磷酸甘油酸激酶、β-actin、蛋白酶体26s亚基、S-腺苷高半胱氨酸水解酶和磷酸核糖酰氨基咪唑羧化酶5个高可信度蛋白.磷酸甘油酸激酶(PGK1)定位于胞质和胞核,在食管癌组织中高表达(69.23%,18/26).结论:改良血清蛋白质组分析策略(mSERPA)可有效分离鉴定肿瘤相关抗原.PGK1是食管癌候选肿瘤相关抗原,在食管癌发生发展中可能发挥重要作用.

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